Document Detail


A new method of separation and quantitation of mucus glycoprotein in rat gastric mucus gel layer and its application to mucus secretion induced by 16,16-dimethyl PGE2.
MedLine Citation:
PMID:  1752389     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method was established for recovering the mucus gel layer of rat gastric mucosa without damage to underlying surface epithelium. The mucus gel was solubilized by stirring the gastric mucosa in a solution of N-acetylcysteine (NAC), a mucolytic agent. Optimal mucus gel solubilization was possible by treatment with 2% NAC for 5 minutes at room temperature. Mucus glycoprotein was quantitatively extracted and measured from the mucus gel sample obtained by the NAC treatment. This treatment caused no damage to surface epithelial cells, as observed by a light microscope. Besides NAC, pronase solution was also adequate for solubilizing the mucus gel layer without any damage to the surface epithelium. However, extraction and measurement of mucus glycoprotein from the pronase-treated mucus gel sample was not possible due to contamination by high molecular hexose-containing substances which were eluted along with the mucus glycoprotein from the column of Bio-Gel A-1.5m. This NAC method was used to examine changes in mucus glycoprotein content in the mucus gel at one hour following the oral administration of 16,16-dimethyl prostaglandin E2. A significant increase in mucus glycoprotein of the gel was brought about by the prostaglandin treatment. Thus, the present method was suitable for estimating the amount of mucus secreted in to the mucus gel layer.
Authors:
Y Komuro; K Ishihara; S Ohara; K Saigenji; K Hotta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gastroenterologia Japonica     Volume:  26     ISSN:  0435-1339     ISO Abbreviation:  Gastroenterol. Jpn.     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1992-01-30     Completed Date:  1992-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0152744     Medline TA:  Gastroenterol Jpn     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  582-7     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Internal Medicine, School of Medicine, Kitasato University, Sagamihara, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Epithelium / drug effects,  pathology
Gastric Mucosa / drug effects*,  pathology
Glycoproteins / analysis*
Male
Mucus / secretion*
Prostaglandins E, Synthetic / pharmacology*
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Prostaglandins E, Synthetic; 0/gastric mucus glycoproteins; 41692-15-3/16,16-dimethylprostaglandin E

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