Document Detail


A new method for the preparation of metaphase chromosomes for flow analysis.
MedLine Citation:
PMID:  6162882     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new method for the preparation of metaphase chromosomes for flow analysis has been evaluated. It has been shown that this method, which involves detergent lysis of metaphase cells and polyamines to stabilize the DNA, yields lower coefficients of variation and background levels in the DNA histograms than is currently obtained by hexylene glycol based methods. A conventional flow cytometer (FACS-II) has been used to resolve the human karyotype into about 14 peaks after ethidium bromide staining and excitation with a relatively low level of illumination (0.4 W at 488 nm). Flow karyotypes have also been obtained from suspension cell lines, in particular from the mouse cell line, Friend 707/B10. The only disadvantage of this method is that the chromosomes are highly condensed and therefore banding studies on sorted chromosomes may not be possible.
Authors:
R Sillar; B D Young
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  29     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-05-21     Completed Date:  1981-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  74-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Cricetinae
Cricetulus
Female
Fetus
Fibroblasts
Humans
Karyotyping / instrumentation*,  methods
Male
Metaphase
Ovary
Staining and Labeling

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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