Document Detail

A new method for mercury removal.
MedLine Citation:
PMID:  16247669     Owner:  NLM     Status:  MEDLINE    
A method is described for the removal of mercury from solution by using the off-gas produced from aerobic cultures of Klebsiella pneumoniae M426. Cells growing in Hg-supplemented medium produced a black precipitate containing mercury and sulphur. The ratio of Hg:S was determined as approximately 1:1 by analysis using proton-induced X-ray emission, suggesting precipitation of HgS within the culture. The outlet gases produced by a mercury-unsupplemented aerated culture were bubbled into an external chamber supplemented with up to 10 mg HgCl(2)/ml. A yellowish-white precipitate formed, corresponding to 99% removal of the mercury from solution within 120 min. Energy dispersive X-ray microanalysis showed that this metal precipitate consisted of mercury, carbon and sulphur. Formation of mercury carbonate was discounted since similar precipitation occurred at pH 2 and no oxygen was detected in the solid, which gave an X-ray powder pattern suggesting an amorphous material, with no evidence of HgS. Precipitation of mercury with a volatile organosulphur compound is suggested. Bio-precipitation of heavy metals by using culture off-gas is a useful approach because it can be used with concentrated or physiologically incompatible solutions. Since the metal precipitate is kept separate from the bacterial biomass, it can be managed independently.
Ashraf M M Essa; Lynne E Macaskie; Nigel L Brown
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology letters     Volume:  27     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-10-25     Completed Date:  2006-01-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1649-55     Citation Subset:  IM    
School of Biosciences, The University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK.
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MeSH Terms
Aerobiosis / physiology
Chemical Precipitation
Klebsiella pneumoniae / growth & development*
Mercury / metabolism*
Water Pollutants / metabolism*
Water Purification / methods
Reg. No./Substance:
0/Water Pollutants; 7439-97-6/Mercury

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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