Document Detail

A new method for measuring oxidative stress in claudicants during strenuous exercise using free radical derivatives of antipyrine as indicators: a pilot study.
MedLine Citation:
PMID:  12017201     Owner:  NLM     Status:  MEDLINE    
Patients with intermittent claudication disease suffer from temporary lack of oxygen in the legs, caused by narrowing of arteries, resulting in ischemia and followed by reperfusion. The degree of oxidative stress present in 16 patients during strenuous exercise was determined using several indicators. Two derivatives of an exogenous marker, antipyrine (AP), (ie, p-hydroxyantipyrine, p-APOH, and o-hydroxyantipyrine, o-APOH), were assayed in plasma using HPLC-tandem-MS. Plasma malondialdehyde (assayed as thiobarbituric acid reactive species, TBARS) was also determined. The branchial/ankle blood pressure index (b-a index) was used to assess the severity of intermittent claudication disease, and plasma lactate concentration was also measured as an indicator of the ischemic situation. Plasma TBARS level did not change significantly after exercise. During the ischemic situation as well as during reperfusion, both free radical derivatives of antipyrine increased significantly in plasma (p < 0.01). Because p-APOH is also formed enzymatically in humans, the plasma ratio of o-APOH to AP appeared to be the most specific marker for oxidative stress in patients with intermittent claudication.
Stefan A J Coolen; Marc H W A Wijnen; Jetse C Reijenga; Huib L Vader; Rudi M H Roumen; Fred A Huf
Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Annals of clinical and laboratory science     Volume:  32     ISSN:  0091-7370     ISO Abbreviation:  Ann. Clin. Lab. Sci.     Publication Date:  2002  
Date Detail:
Created Date:  2002-05-17     Completed Date:  2002-11-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0410247     Medline TA:  Ann Clin Lab Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  181-7     Citation Subset:  IM    
University of Technology Eindhoven, Laboratory of Instrumental Analysis, The Netherlands.
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MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / diagnostic use*,  pharmacokinetics
Antipyrine / diagnostic use*,  pharmacokinetics
Free Radicals / metabolism
Intermittent Claudication / diagnosis*,  metabolism*
Lactic Acid / blood
Malondialdehyde / blood
Middle Aged
Oxidative Stress*
Pilot Projects
Thiobarbituric Acid Reactive Substances / metabolism
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Free Radicals; 0/Thiobarbituric Acid Reactive Substances; 50-21-5/Lactic Acid; 542-78-9/Malondialdehyde; 60-80-0/Antipyrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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