| A new method for measuring oxidative stress in claudicants during strenuous exercise using free radical derivatives of antipyrine as indicators: a pilot study. | |
MedLine Citation:
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PMID: 12017201 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Patients with intermittent claudication disease suffer from temporary lack of oxygen in the legs, caused by narrowing of arteries, resulting in ischemia and followed by reperfusion. The degree of oxidative stress present in 16 patients during strenuous exercise was determined using several indicators. Two derivatives of an exogenous marker, antipyrine (AP), (ie, p-hydroxyantipyrine, p-APOH, and o-hydroxyantipyrine, o-APOH), were assayed in plasma using HPLC-tandem-MS. Plasma malondialdehyde (assayed as thiobarbituric acid reactive species, TBARS) was also determined. The branchial/ankle blood pressure index (b-a index) was used to assess the severity of intermittent claudication disease, and plasma lactate concentration was also measured as an indicator of the ischemic situation. Plasma TBARS level did not change significantly after exercise. During the ischemic situation as well as during reperfusion, both free radical derivatives of antipyrine increased significantly in plasma (p < 0.01). Because p-APOH is also formed enzymatically in humans, the plasma ratio of o-APOH to AP appeared to be the most specific marker for oxidative stress in patients with intermittent claudication. |
Authors:
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Stefan A J Coolen; Marc H W A Wijnen; Jetse C Reijenga; Huib L Vader; Rudi M H Roumen; Fred A Huf |
Publication Detail:
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Type: Clinical Trial; Journal Article |
Journal Detail:
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Title: Annals of clinical and laboratory science Volume: 32 ISSN: 0091-7370 ISO Abbreviation: Ann. Clin. Lab. Sci. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-05-17 Completed Date: 2002-11-22 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0410247 Medline TA: Ann Clin Lab Sci Country: United States |
Other Details:
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Languages: eng Pagination: 181-7 Citation Subset: IM |
Affiliation:
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University of Technology Eindhoven, Laboratory of Instrumental Analysis, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Anti-Inflammatory Agents, Non-Steroidal / diagnostic use*, pharmacokinetics Antipyrine / diagnostic use*, pharmacokinetics Exercise* Female Free Radicals / metabolism Humans Hydroxylation Intermittent Claudication / diagnosis*, metabolism* Lactic Acid / blood Male Malondialdehyde / blood Middle Aged Oxidative Stress* Pilot Projects Thiobarbituric Acid Reactive Substances / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Free Radicals; 0/Thiobarbituric Acid Reactive Substances; 50-21-5/Lactic Acid; 542-78-9/Malondialdehyde; 60-80-0/Antipyrine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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