Document Detail

A new method of the measurement of the electrically neutral fluxes of cations through lipid bilayer membranes induced by Men+/nH+-exchangers.
MedLine Citation:
PMID:  6414844     Owner:  NLM     Status:  MEDLINE    
Electrically neutral ionophores (nigericin, monencin) incorporated into a planar bilayer lipid membrane (BLM) bring about hydrogen ion gradient formation in the unstirred layers of BLM if a metal ion gradient on the membrane is prepared. Under these conditions a diffusion potential of a hydrogen ion is generated after addition of a protonophore. Cation selectivity of nigericin, monencin and A23187 has been studied by means of electrical potential measurements in the presence of a protonophore and Men+/nH+-exchangers mentioned above. The data on cation selectivity are in a good agreement with the well known results of the direct measurements of metal ion fluxes. This shows that the effect of generation of the potential on BLM in the presence of a protonophore and a Men+/nH+-exchanger can be used for the estimation of electrically neutral ion fluxes through BLM.
Y N Antonenko; L S Yaguzhinsky
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  163     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1983-12-17     Completed Date:  1983-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  42-5     Citation Subset:  IM    
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MeSH Terms
Anti-Bacterial Agents
Hydrogen-Ion Concentration
Lipid Bilayers*
Membrane Potentials
Models, Biological
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Cations; 0/Ionophores; 0/Lipid Bilayers; 17090-79-8/Monensin; 28380-24-7/Nigericin; 52665-69-7/Calcimycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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