Document Detail

A new method for isolating large quantities of Arabidopsis trichomes for transcriptome, cell wall and other types of analyses.
MedLine Citation:
PMID:  18643981     Owner:  NLM     Status:  MEDLINE    
A new procedure has been developed for the isolation of wild-type and mutant Arabidopsis trichomes. The isolated trichomes maintained enzymatic activity and were used for DNA, protein, and RNA isolation. The RNA was used to generate probes suitable for Affymetrix analysis. The validity of the Affymetrix results was confirmed by quantitative PCR analysis on a subset of genes that are preferentially expressed in trichomes or leaves. Sufficient quantities of trichomes were isolated to probe the biochemical nature of trichome cell walls. These analyses provide evidence for the presence of lignin in Arabidopsis trichome cell walls. The monosaccharide analysis and positive staining with ruthenium red indicates that the walls also contain a large portion of pectin. The 2.23-fold ratio of pectin-related sugars compared with potential cellulosic glucose suggests that the polysaccharides of the trichome cell walls are more like those of typical primary walls even though the wall becomes quite thick. Overall, these analyses open the door to using the Arabidopsis trichome cell wall as an excellent model to probe various questions concerning plant cell wall biosynthesis.
M David Marks; Lissete Betancur; Edward Gilding; Fang Chen; Stefan Bauer; Jonathan P Wenger; Richard A Dixon; Candace H Haigler
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-04
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  56     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-23     Completed Date:  2008-11-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  483-92     Citation Subset:  IM    
Department of Plant Biology, University of Minnesota, 1445 Gortner Ave., St Paul, MN 55108, USA.
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MeSH Terms
Arabidopsis / chemistry,  cytology*,  genetics
Arabidopsis Proteins / isolation & purification
Cell Separation / methods*
Cell Wall / chemistry*
DNA, Plant / genetics,  isolation & purification
Gene Expression Profiling*
Genes, Plant
Genome, Plant
Lignin / chemistry
Microscopy, Electron, Transmission
Monosaccharides / chemistry
Plant Epidermis / cytology,  genetics
Plant Leaves / cytology,  genetics
Polymerase Chain Reaction
RNA, Plant / genetics,  isolation & purification
Reg. No./Substance:
0/Arabidopsis Proteins; 0/DNA, Plant; 0/Monosaccharides; 0/RNA, Plant; 9005-53-2/Lignin

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