| [A new method of identifying the peripheral blood and the menstrual blood]. | |
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MedLine Citation:
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PMID: 23213787 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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OBJECTIVE: To explore the tissue-specific gene expressions of the peripheral blood and the menstrual blood, and to search some specific factors to establish an effective method for identifying the peripheral blood and the menstrual blood. METHODS: The specific products of the peripheral blood and the menstrual blood were detected by RT-PCR and separated by electrophoretic technology. RESULTS: Beta-spectrin (SPTB) as one specific marker of peripheral blood and 18S rRNA as a kind of the housekeeping gene were expressed in both the peripheral blood and the menstrual blood. However, matrix metalloproteinase 7 (MMP7) as one specific marker of menstrual blood and human beta defensin 1 (HBD1) as one specific marker of vaginal discharge were only found in the menstrual blood. CONCLUSION: There are differences of specific gene expressions between the peripheral blood and the menstrual blood. They could be accurately distinguished from each other by using the combination of fluorescence technology and RT-PCR to detect the specific identification of mRNA. |
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Authors:
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Ying-xi Wang; Xiao-jun Zhu; Zhang-ping Jiao; Zhi-yong Lu; Wei-ni Wang; Ya-cheng Liu; Hui Tang |
Publication Detail:
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Type: English Abstract; Journal Article |
Journal Detail:
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Title: Fa yi xue za zhi Volume: 28 ISSN: 1004-5619 ISO Abbreviation: Fa Yi Xue Za Zhi Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-12-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9426151 Medline TA: Fa Yi Xue Za Zhi Country: China |
Other Details:
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Languages: chi Pagination: 359-61 Citation Subset: IM |
Affiliation:
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School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. wangyingxi4722@163.com |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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