Document Detail


[A new method of identifying the peripheral blood and the menstrual blood].
MedLine Citation:
PMID:  23213787     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
OBJECTIVE: To explore the tissue-specific gene expressions of the peripheral blood and the menstrual blood, and to search some specific factors to establish an effective method for identifying the peripheral blood and the menstrual blood.
METHODS: The specific products of the peripheral blood and the menstrual blood were detected by RT-PCR and separated by electrophoretic technology.
RESULTS: Beta-spectrin (SPTB) as one specific marker of peripheral blood and 18S rRNA as a kind of the housekeeping gene were expressed in both the peripheral blood and the menstrual blood. However, matrix metalloproteinase 7 (MMP7) as one specific marker of menstrual blood and human beta defensin 1 (HBD1) as one specific marker of vaginal discharge were only found in the menstrual blood.
CONCLUSION: There are differences of specific gene expressions between the peripheral blood and the menstrual blood. They could be accurately distinguished from each other by using the combination of fluorescence technology and RT-PCR to detect the specific identification of mRNA.
Authors:
Ying-xi Wang; Xiao-jun Zhu; Zhang-ping Jiao; Zhi-yong Lu; Wei-ni Wang; Ya-cheng Liu; Hui Tang
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Fa yi xue za zhi     Volume:  28     ISSN:  1004-5619     ISO Abbreviation:  Fa Yi Xue Za Zhi     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-12-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426151     Medline TA:  Fa Yi Xue Za Zhi     Country:  China    
Other Details:
Languages:  chi     Pagination:  359-61     Citation Subset:  IM    
Affiliation:
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. wangyingxi4722@163.com
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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