Document Detail


A new method for encapsulation of living cells: preliminary results with PC12 cell line.
MedLine Citation:
PMID:  11308223     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new method is described for encapsulation of living cells. PC12 rat adrenal pheochromocytoma cells, which have been shown to synthesize, store and release dopamine were employed. The particles are made first and the cells then incorporated in a gentle mechanical procedure. The morphology (by light and electron microscopic observation), stability, rheology, texture and permeability of these microcapsules provided by Kappa Biotech were investigated. Membrane permeability studies demonstrated exclusion of 69,000 Da human serum albumin, but equilibrium of D-glucose and inulin was within 24h, indicating a molecular weight cut-off in the 5000-70,000 Da range. The viability and the function of the encapsulated cells were evaluated by measuring the spontaneous release of dopamine by high performance liquid chromatography with electrochemical detection. The results show that dopamine-secreting cells can be sequestered in a semi-permeable capsule and still display good viability and proliferation for at least 1 month.
Authors:
P Mercier; F Fernandez; F Tortosa; H Bagheri; H Duplan; M Tafani; J C Bes; R Bastide; Y Lazorthes; B Sallerin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microencapsulation     Volume:  18     ISSN:  0265-2048     ISO Abbreviation:  J Microencapsul     Publication Date:    2001 May-Jun
Date Detail:
Created Date:  2001-04-18     Completed Date:  2001-09-06     Revised Date:  2009-07-21    
Medline Journal Info:
Nlm Unique ID:  8500513     Medline TA:  J Microencapsul     Country:  England    
Other Details:
Languages:  eng     Pagination:  323-34     Citation Subset:  IM    
Affiliation:
Société Kappa Biotech, Montauban, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biodegradation, Environmental
Cell Division
Cell Survival
Cells, Immobilized
Dopamine / secretion
Drug Compounding / methods*
Humans
Immunohistochemistry
Microscopy, Electron, Scanning
Microspheres
PC12 Cells
Particle Size
Permeability
Rats
Time Factors
Tyrosine 3-Monooxygenase / metabolism
Chemical
Reg. No./Substance:
EC 1.14.16.2/Tyrosine 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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