| A new method for the determination of nucleic acid using an Eu3+- nicotinic acid complex as a resonance light scattering probe. | |
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MedLine Citation:
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PMID: 19104482 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study found that in Tris-HCl buffer, the resonance light scattering (RLS) intensity of the Eu(3+)-nicotinic acid system can be greatly enhanced by nucleic acids and the enhanced intensity is proportional to the concentration of nucleic acid in the range of 7 x 10(-8)-1 x 10(-5 ) g x mL(-1) for fsDNA, and its detection limit is 2 x 10(-8 ) g x mL(-1). Based on this, a new method for the determination of nucleic acids is proposed. Synthetic and actual samples are determined satisfactorily. The interaction mechanism is also studied. It is thought that nucleic acid can bind with the Eu(3+)-nicotinic acid complex through electrostatic attraction and thus form a large Eu(3+)-nicotinic acid-nucleic acid complex. |
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Authors:
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Meng Guo; Lin-Tong Wang; Xia Wu; Wei Xu; Jing-He Yang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-23 |
Journal Detail:
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Title: Molecules (Basel, Switzerland) Volume: 14 ISSN: 1420-3049 ISO Abbreviation: Molecules Publication Date: 2009 |
Date Detail:
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Created Date: 2008-12-23 Completed Date: 2009-01-30 Revised Date: 2009-10-27 |
Medline Journal Info:
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Nlm Unique ID: 100964009 Medline TA: Molecules Country: Switzerland |
Other Details:
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Languages: eng Pagination: 10-8 Citation Subset: IM |
Affiliation:
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Microscale Science Institute, Department of Chemistry, Weifang University, Weifang 261061, PR China. guomeng7101@126.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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analysis* Europium / chemistry* Light Methods Niacin / chemistry* RNA / analysis* Scattering, Radiation* Tromethamine |
| Chemical | |
Reg. No./Substance:
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59-67-6/Niacin; 63231-63-0/RNA; 7440-53-1/Europium; 77-86-1/Tromethamine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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