| A new method of biosensing with 1 microl of Escherichia coli suspension using atomic force microscopy. | |
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MedLine Citation:
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PMID: 16125129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed a new method for detecting bacterial cells from 1-mul samples with atomic force microscopy (AFM). The use of a parafilm surface as a sample palette was effective for reacting small amounts of samples with an AFM probe. This was due to the parafilm's hydrophobic, semitransparent, and nonadhesive surface. In this way, all processes, such as the surface functionalization of a cantilever and the adhesion of Escherichia coli cells to a cantilever, were easily completed. In addition, we succeeded in detecting cell adsorption on the same AFM cantilever by both the drive mode and the thermal mode. The resonance frequency shift caused by cell adhesion was clearly detected by the two modes for the first time. Our data indicated the potential of applying AFM nanobiosensing to extremely small amounts of samples. |
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Authors:
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Satoshi Tanaka; Hiroaki Sugasawa; Takashi Morii; Takao Okada; Masanori Abe; Noriaki Kato; Reiko Kuroda; Taishi Nasu; Masayuki Nagai; Kazuo Umemura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 345 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-09-26 Completed Date: 2005-12-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 116-21 Citation Subset: IM |
Affiliation:
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Faculty of Engineering, Musashi Institute of Technology, 1-28-1 Tamazutsumi, Setagaya, Tokyo 158-8557, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Adhesion Biosensing Techniques* / methods Escherichia coli* Microscopy, Atomic Force* / methods Nanotechnology* / methods Sample Size Surface Properties |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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