Document Detail


A new, less toxic polymerization system for the embedding of soft tissues in glycol methacrylate and subsequent preparing of serial sections.
MedLine Citation:
PMID:  6361264     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The paper describes a new polymerization system for embedding soft tissues in glycol methacrylate (GMA). The polymerization of GMA is initiated by means of a barbituric acid derivative in combination with chloride ions and dibenzoyl peroxide. The catalyst system contains no aromatic amines which constitutes a toxicological advantage over the commonly employed system of peroxide/aromatic amine. Clear blocks are obtained from which 1-2 micrometers sections are easy to cut. In combination with an appropriate softener, polyethylene glycol 400, serial sectioning may be practiced.
Authors:
P O Gerrits; L Smid
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  132     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1984-02-24     Completed Date:  1984-02-24     Revised Date:  2006-08-08    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  81-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acrylates*
Animals
Barbiturates
Histological Techniques*
Methacrylates*
Polyethylene Glycols
Polymers
Rats
Chemical
Reg. No./Substance:
0/Acrylates; 0/Barbiturates; 0/Methacrylates; 0/Polyethylene Glycols; 0/Polymers; 67-52-7/barbituric acid; 868-77-9/hydroxyethyl methacrylate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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