Document Detail

A new label-free amperometric immunosenor for rubella vaccine.
MedLine Citation:
PMID:  15761742     Owner:  NLM     Status:  MEDLINE    
A novel label-free amperometric immunosensor for the detection of rubella vaccine was developed by immobilizing anti-rubella serum on bilayer nano-Au/polymerized o-phenylenediamine film with electrodeposited Prussian Blue (PB) as an electrode transfer mediator on the platinum electrode. The redox reactions of PB as a probe on the platinum surface were blocked due to the binding of the antibody to the antigen, which was investigated by cyclic voltammetry. Therefore, the interaction of the antibody with various concentrations of antigen could be detected by measurements of amperometric response in PBS, and the amperometric response on the surface of the modified electrode was inversely proportional to the concentration of rubella vaccine in the sample. The immunosensor showed a specific response to rubella vaccine in the range 8.1x10(-8)-8.0x10(-6) lgCCID50/ml (cell culture infectious dose) and a detection limit of 4.010(-8) lgCCID50/ml at a signal-to-noise ratio of 3. To summarize, the present work provides a low-cost, fast response time, highly sensitive and easy-to-prepare method for the determination of antigen in biological products.
Lingyan Zhang; Ruo Yuan; Xiaoqing Huang; Yaqin Chai; Dianping Tang; Shurui Cao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-27
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  381     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-11     Completed Date:  2005-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1036-40     Citation Subset:  IM    
Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing, 400715, China.
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MeSH Terms
Antibodies, Viral / blood
Antigens, Viral / analysis*
Biosensing Techniques* / instrumentation
Ferrocyanides / chemistry
Gold / chemistry
Phenylenediamines / chemistry
Rubella Vaccine / analysis*,  immunology
Surface Properties
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral; 0/Ferrocyanides; 0/Phenylenediamines; 0/Rubella Vaccine; 14038-43-8/ferric ferrocyanide; 7440-57-5/Gold

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