Document Detail

A new in vitro model for analyzing the biological behavior of well-differentiated squamous cell carcinoma.
MedLine Citation:
PMID:  15807308     Owner:  NLM     Status:  MEDLINE    
A suitable model analyzing the behavior of well-differentiated squamous cell carcinoma has not yet been established. We tried to establish such a system using a reconstructed oral mucosa, in which T3M-1 squamous cell carcinoma cells were cultured on 3T3 fibroblast-containing collagen gel. Fibroblasts promoted the stratification and keratinization of T3M-1 cells. During growth, the Ki-67 index of T3M-1 cells with fibroblasts was higher than that of T3M-1 cells alone. Fibroblasts increased the expression of involucrin, a differentiating marker of keratinocytes, in T3M-1 cells. They also promoted the invasion of T3M-1 cells into the gel. When T3M-1 cells alone were cultured in a fibroblast-conditioned (FC) medium, the fibroblast-induced phenomena mentioned above were almost replicated. In addition, epidermal growth factqr (EGF) promoted T3M-1 cells growth, but not the invasion. cDNA microarray analysis showed that FC medium increased the expression of EGF receptor and several other mRNAs of T3M-1 cells. The data suggest that T3M-1 cells, under cancer-stromal fibroblast interaction, undergo invasive growth with their well-differentiated squamous phenotype, and that this interaction may be mediated partly by soluble molecules (e.g., EGF) in an autocrine or paracrine pathway. Our system will probably provide a useful model for analyzing the biological behavior of well-differentiated squamous cell carcinoma.
Shintaro Satoh; Shuji Toda; Akira Inokuchi; Hajime Sugihara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pathology, research and practice     Volume:  201     ISSN:  0344-0338     ISO Abbreviation:  Pathol. Res. Pract.     Publication Date:  2005  
Date Detail:
Created Date:  2005-04-05     Completed Date:  2005-06-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806109     Medline TA:  Pathol Res Pract     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  27-35     Citation Subset:  IM    
Department of Pathology and Biodefense, Division of Cellular and Molecular Pathology, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
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MeSH Terms
3T3 Cells
Carcinoma, Squamous Cell / metabolism,  pathology*,  physiopathology*
Cell Differentiation
Cell Division / drug effects
Coculture Techniques
Culture Media, Conditioned / pharmacology
Epidermal Growth Factor / pharmacology
Hepatocyte Growth Factor / pharmacology
Ki-67 Antigen / metabolism
Mouth Neoplasms / metabolism,  pathology*,  physiopathology*
Neoplasm Invasiveness
Oligonucleotide Array Sequence Analysis
Protein Precursors / metabolism
Receptors, Interleukin-1 / antagonists & inhibitors
Stromal Cells
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Gels; 0/Ki-67 Antigen; 0/Protein Precursors; 0/Receptors, Interleukin-1; 60108-77-2/involucrin; 62229-50-9/Epidermal Growth Factor; 67256-21-7/Hepatocyte Growth Factor; 9007-34-5/Collagen

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