Document Detail


A new immunoassay for the measurement of myoglobin in serum.
MedLine Citation:
PMID:  8189324     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Because the concentration of serum myoglobin (Mb) increases within 2 to 4 hours after the first sign of acute myocardial infarction, it has been proposed as an early marker of the condition. Our aim was to evaluate a new assay that provides a rapid, quantitative determination of Mb (Baxter Stratus Myoglobin) based on the radial partition technique. We compared the results obtained by this technique with those from nephelometric and radioimmunoassay methods. A significant agreement was observed, the correlation coefficients (r) being 0.999 and 0.996, respectively. The method evaluated provided good reproducibility with CVs between 3.14% and 4.87%, and its linearity and analytical sensitivity were satisfactory. The clinical evaluation of this assay demonstrates that Mb increases in serum of patients with acute myocardial infarction before total creatine kinase and creatine kinase MB isoenzyme. Mb concentration shows an early peak and earlier return to normal values after the necrosis compared to enzymatic activities. Moreover the assay is rapid and fully automated. The method is therefore considered appropriate for contributing to the early diagnosis of AMI in clinical laboratories.
Authors:
P Carraro; M Plebani; M C Varagnolo; M Zaninotto; M Rossetti; A Burlina
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical laboratory analysis     Volume:  8     ISSN:  0887-8013     ISO Abbreviation:  J. Clin. Lab. Anal.     Publication Date:  1994  
Date Detail:
Created Date:  1994-06-23     Completed Date:  1994-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8801384     Medline TA:  J Clin Lab Anal     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  70-5     Citation Subset:  IM    
Affiliation:
Institute of Laboratory Medicine, University of Padua, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Child
Child, Preschool
Creatine Kinase / blood
Female
Humans
Immunoenzyme Techniques* / statistics & numerical data
Isoenzymes
Latex Fixation Tests
Male
Middle Aged
Myocardial Infarction / blood,  diagnosis,  enzymology
Myoglobin / blood*
Radioimmunoassay
Reference Values
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Myoglobin; EC 2.7.3.2/Creatine Kinase

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