| A new genetic method to generate and isolate small, short-lived but highly potent dendritic cell-tumor cell hybrid vaccines. | |
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MedLine Citation:
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PMID: 12925849 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fusion of tumor cells with antigen-presenting cells (APCs) has been proposed for the preparation of cancer vaccines. However, generation of these hybrids, using physical or chemical methods such as electrofusion or polyethylene glycol (PEG), has been difficult to standardize. Characterization of cell fusion has also been problematic because of difficulties in differentiating fusion from cell aggregation, leakage of cellular dyes and dendritic-cell (DC) phagocytosis of tumor material. In this report, we describe a new method to generate hybrid cell vaccines, based on gene transfer of a viral fusogenic membrane glycoprotein (FMG) into tumor cells, and incorporate a genetic method by which true hybrid formation can be unambiguously detected. We describe a new class of tumor cell-DC hybrid that can be rapidly isolated after cell fusion. These hybrids are highly potent in in vitro antigen presentation assays, target lymph nodes in vivo and are powerful immunogens against established metastatic disease. |
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Authors:
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Vy Phan; Fiona Errington; S Chiat Cheong; Tim Kottke; Michael Gough; Sharon Altmann; Annick Brandenburger; Steve Emery; Scott Strome; Andrew Bateman; Bernard Bonnotte; Alan Melcher; Richard Vile |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2003-08-17 |
Journal Detail:
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Title: Nature medicine Volume: 9 ISSN: 1078-8956 ISO Abbreviation: Nat. Med. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-09-01 Completed Date: 2003-11-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9502015 Medline TA: Nat Med Country: United States |
Other Details:
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Languages: eng Pagination: 1215-9 Citation Subset: IM |
Affiliation:
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Molecular Medicine Program, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Neoplasm Cancer Vaccines / genetics*, immunology, pharmacology Cell Fusion / methods Dendritic Cells / cytology*, physiology Genetic Techniques* Glycoproteins / genetics Hybrid Cells Lymph Nodes / pathology Melanoma / genetics, pathology, therapy Mice Mice, Inbred C57BL Receptors, Antigen, T-Cell / genetics, immunology, metabolism T-Lymphocytes / immunology Tumor Cells, Cultured Vaccination |
| Grant Support | |
ID/Acronym/Agency:
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P50 CA91956/CA/NCI NIH HHS; R01 CA094180/CA/NCI NIH HHS; R01 CA85931/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Neoplasm; 0/Cancer Vaccines; 0/Glycoproteins; 0/Receptors, Antigen, T-Cell |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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