Document Detail


A new general solid-phase method for the synthesis of backbone-to-backbone cyclized peptides.
MedLine Citation:
PMID:  8070975     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A model peptide with the sequences Ala-Pro-Lys(2ClZ)-Tyr(2BrZ) was synthesized on a 4-methylbenzhydryl amine (MBHA) polystyrene resin using conventional Boc/benzyl protective group strategy. The amino acid aldehyde Boc-valinal was coupled by reductive alkylation with NaCNBH3 in acidified DMF for 1 h. The secondary amine in the peptide-resin Boc-Val psi[CH2NH]Ala-Pro-Lys(2ClZ)-Tyr(2BrZ)-MBHA was reductively alkylated by 3(4-methylbenzylthio)-propanal at 40 degrees C for 6 h, resulting the peptide-resin Boc-Val psi[CH2N(CH2CH2CH2-S-pMeBzl)]Ala-Pro-Lys(2ClZ)-T yr(2BrZ)-MBHA. After the removal of the Boc group the synthesis was continued employing the above-mentioned methods, which led to the resin-bound peptide Leu psi[CH2N(CH2CH2CH2S-pMeBzl)]Ser-Pro-Gly-Lys(2ClZ )-Val psi [CH2N(CH2CH2CH2-S-pMeBzl)]Ala-Pro-Lys(2ClZ)-Tyr( 2BrZ)-MBHA. The peptide was cleaved from the resin with hydrogen fluoride. Reversed-phase HPLC and plasma desorbtion mass spectrometry analysis showed that the expected peptide Leu psi[CH2N(CH2CH2CH2SH)]Ser-Pro-Gly-Lys-Val psi[CH2N(CH2CH2CH2-SH)] Ala-Pro-Lys-Tyr-NH2 was obtained as the major product with low levels of side products. Intramolecular oxidation of the thiols gave the backbone to backbone cyclized peptide Leu psi[CH2N(CH2CH2CH2S)]Ser-Pro-Gly-Lys- Val psi[CH2N(CH2CH2CH2-S)]Ala-Pro-Lys-Tyr-NH2.
Authors:
K Kaljuste; A Undén
Related Documents :
6198465 - Cleavage of rabbit myelin basic protein by thrombin.
6777325 - Cyclic peptides. viii. synthesis and tryptic hydrolysis of cyclic depsidipeptides conta...
1823925 - Primary structure and relative potency of an analog of beta-pdh (pigment-dispersing hor...
8500915 - Purification and characterization of streptolysin o secreted by streptococcus equisimil...
9609235 - Preparation of a novel streptokinase mutant with improved stability.
23441895 - Mechanistic aspects of lanthipeptide leaders.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of peptide and protein research     Volume:  43     ISSN:  0367-8377     ISO Abbreviation:  Int. J. Pept. Protein Res.     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-09-27     Completed Date:  1994-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0330420     Medline TA:  Int J Pept Protein Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  505-11     Citation Subset:  IM    
Affiliation:
Department of Neurochemistry and Neurotoxicology, Stockholm University, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alkylation
Amino Acid Sequence
Chromatography, High Pressure Liquid
Molecular Sequence Data
Peptides, Cyclic / chemical synthesis*,  isolation & purification
Chemical
Reg. No./Substance:
0/Peptides, Cyclic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Substrate phosphorylation capacities of the major tyrosine protein kinase from the human promyelocyt...
Next Document:  Referral patterns of dentists: transference-countertransference.