Document Detail

A new factor derived from 1321N1 human astrocytoma cells causes differentiation of PC-12 cells mediated through mitogen-activated protein kinase cascade.
MedLine Citation:
PMID:  9739111     Owner:  NLM     Status:  MEDLINE    
Glial cells play an important role in maintaining neural function. In the present study, we examined the effects of a factor derived from human astrocytoma cells (1321N1) on differentiation of rat pheochromocytoma cells (PC-12). The conditioned medium which had been used for culture of 1321N1 cells caused the differentiation of PC-12 cells, suggesting that 1321N1 cells release a neurotrophic factor. The factor was apparently distinct from well-known neurotrophic factors, such as nerve growth factor (NGF), since it was resistant to boiling and trypsin treatment. The molecular size of the factor was assumed to be below 1000 through dialysis and ultrafiltration experiments. Furthermore, PC-12 cells were differentiated synergistically by the combined addition of NGF and the conditioned medium of 1321N1 cells. Partially purified fraction of the factor by Sephadex G-15 gel filtration column caused the prolonged activation of mitogen-activated protein kinase (MAPK). The differentiation of PC-12 cells induced by the fraction or NGF disappeared after the treatment with PD98059, a specific inhibitor of MAPK kinase (MEK), suggesting the involvement of MAPK in the differentiation. These results suggest that the new low-molecular factor derived from glial cells causes differentiation of PC-12 cells mediated through an activation of MAPK.
Y Obara; N Nakahata; Y Ohizumi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  806     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-11-20     Completed Date:  1998-11-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  79-88     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Elsevier Science B.V.
Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
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MeSH Terms
Astrocytoma / chemistry*
Calcium-Calmodulin-Dependent Protein Kinases / metabolism*,  physiology
Cell Differentiation / physiology
Cell Survival / drug effects
Culture Media, Conditioned / chemistry,  pharmacology
PC12 Cells / drug effects*,  pathology*
Tissue Extracts / chemistry,  pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Tissue Extracts; EC Protein Kinases

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