Document Detail


A new direct Fourier transform infrared analysis of free fatty acids in edible oils using spectral reconstitution.
MedLine Citation:
PMID:  21556753     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A new transmission-based Fourier transform infrared (FTIR) spectroscopic method for the direct determination of free fatty acids (FFA) in edible oils has been developed using the developed spectral reconstitution (SR) technique. Conventional neat-oil and SR calibrations were devised by spiking hexanoic acid into FFA-free canola oil and measuring the response to added FFA at 1,712 cm(-1) referenced to a baseline at 1,600 cm(-1)(1,712 cm(-1)/1,600 cm(-1)). To compensate for the known oil dependency of such calibration equations resulting from variation of the triacylglycerol ester (C═O) absorption with differences in oil saponification number (SN), a correction equation was devised by recording the spectra of blends of two FFA-free oils (canola and coconut) differing substantially in SN and correlating the intensity of the ester (C═O) absorption at the FFA measurement location with the intensity of the first overtone of this vibration, measured at 3,471 cm(-1)/3,427 cm(-1). Further examination of the spectra of the oil blends by generalized 2D correlation spectroscopy revealed an additional strong correlation with an absorption in the near-infrared (NIR) combination band region, which led to the development of a second correction equation based on the absorbance at 4,258 cm(-1)/4,235 cm(-1). The NIR-based correction equation yielded superior results and was shown to completely eliminate biases due to variations in oil SN, thereby making a single FFA calibration generally applicable to oils, regardless of SN. FTIR methodology incorporating this correction equation and employing the SR technique has been automated.
Authors:
Xiuzhu Yu; F R van de Voort; Jacqueline Sedman; Jin-Ming Gao
Related Documents :
1435093 - Effects of dietary linseed oil and marine oil on lipid peroxidation in monkey liver in ...
20688263 - Fish oil supplementation increases the cyclooxygenase inhibitory activity of paracetamo...
6929083 - Effects of chlorhexidine and iodine on in vitro plaques of streptococcus mutans and str...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-10
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  -     ISSN:  1618-2650     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road Yangling, 712100, Shaanxi, China, xiuzhuyu1004@hotmail.com.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Lipid analysis of flat-mounted eye tissue by imaging mass spectrometry with identification of contam...
Next Document:  Unpredictability of metabolism--the key role of metabolomics science in combination with next-genera...