Document Detail

A new differentiation antigen (FT-1) shared with fetal thymocytes and leukemic cells in the mouse.
Jump to Full Text
MedLine Citation:
PMID:  6608576     Owner:  NLM     Status:  MEDLINE    
A mouse monoclonal antibody (IgM) was obtained by cell hybridization between X63-Ag8.653 myeloma cells and spleen cells from a BALB mouse that was immunized with GRSL leukemia cells of the GR strain. This antibody identified a unique fetal antigen, which is expressed exclusively on embryonic thymocytes of all strains tested. Therefore, the antigen defined was named fetal thymus antigen-1, FT-1. The proportion of FT-1+ fetal thymocytes detected by immunofluorescence assay sharply decreases as gestation time increases, and finally they disappear from the thymus. On the other hand, Thy-1+ cells increase in inverse proportion. The immunofluorescence studies and absorption tests showed that FT-1 antigen is not detectable on brain, liver, kidney, or lymphoid tissue cells of adult mice. However, it is expressed on some leukemia cells of various mouse strains, which demonstrated that this is the first example of an oncofetal antigen of a mouse leukemia. The molecular weight of FT-1 antigen on leukemia cells was estimated to be 130,000 by means of biosynthetic labeling with [3H]galactose and [35S]methionine. The two-dimensional gel electrophoresis pattern of FT-1 antigen shows a family of glycoproteins with extensive charge heterogeneity. It was also shown that the FT-1 antigen molecule carries the receptor for DBA lectin.
M Kasai; T Takashi; T Takahashi; T Tokunaga
Related Documents :
24637076 - Infection and autoimmunity in sjogren's syndrome: a clinical study and comprehensive re...
3219236 - Htlv-1 infection in tropical spastic paraparesis: lymphocyte culture and serologic resp...
3873306 - Chronic b-cell leukemias: relation between morphological and immunological features.
11817066 - Flow cytometric and immunophenotypic evaluation of acute lymphocytic leukemia in dog bo...
1601036 - Immunity after treatment of human schistosomiasis: association between ige antibodies t...
9928876 - Prevalence of igm and igg antibodies to toxoplasma gondii in blood donors in the czech ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  159     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-05-14     Completed Date:  1984-05-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  971-80     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibodies, Monoclonal / immunology
Antigens, Neoplasm / immunology,  isolation & purification*
Antigens, Surface / immunology,  isolation & purification*
Cell Differentiation
Cell Line
Lectins / immunology
Leukemia, Experimental / immunology*
Membrane Glycoproteins*
Mice, Inbred A
Mice, Inbred AKR
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Inbred DBA
Mice, Inbred NZB
Molecular Weight
Receptors, Mitogen / isolation & purification
T-Lymphocytes / cytology,  immunology*,  pathology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/Antigens, Surface; 0/Dolichos biflorus lectin receptor; 0/Lectins; 0/Membrane Glycoproteins; 0/Receptors, Mitogen; 0/thymus-leukemia antigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
Download PDF

Print publication date: Day: 1 Month: 4 Year: 1984
Volume: 159 Issue: 4
First Page: 971 Last Page: 980
ID: 2187277
Publisher Id: 84163781
PubMed Id: 6608576

A new differentiation antigen (FT-1) shared with fetal thymocytes and leukemic cells in the mouse

Article Categories:
  • Articles

Previous Document:  Induction of idiotope suppression in the anti-azophenylarsonate response of T-depleted A/J mice.
Next Document:  Phorbol myristate acetate induction of lymphotoxins from continuous human B lymphoid cell lines in v...