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A new differentiation antigen (FT-1) shared with fetal thymocytes and leukemic cells in the mouse.
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MedLine Citation:
PMID:  6608576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mouse monoclonal antibody (IgM) was obtained by cell hybridization between X63-Ag8.653 myeloma cells and spleen cells from a BALB mouse that was immunized with GRSL leukemia cells of the GR strain. This antibody identified a unique fetal antigen, which is expressed exclusively on embryonic thymocytes of all strains tested. Therefore, the antigen defined was named fetal thymus antigen-1, FT-1. The proportion of FT-1+ fetal thymocytes detected by immunofluorescence assay sharply decreases as gestation time increases, and finally they disappear from the thymus. On the other hand, Thy-1+ cells increase in inverse proportion. The immunofluorescence studies and absorption tests showed that FT-1 antigen is not detectable on brain, liver, kidney, or lymphoid tissue cells of adult mice. However, it is expressed on some leukemia cells of various mouse strains, which demonstrated that this is the first example of an oncofetal antigen of a mouse leukemia. The molecular weight of FT-1 antigen on leukemia cells was estimated to be 130,000 by means of biosynthetic labeling with [3H]galactose and [35S]methionine. The two-dimensional gel electrophoresis pattern of FT-1 antigen shows a family of glycoproteins with extensive charge heterogeneity. It was also shown that the FT-1 antigen molecule carries the receptor for DBA lectin.
Authors:
M Kasai; T Takashi; T Takahashi; T Tokunaga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  159     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-05-14     Completed Date:  1984-05-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  971-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Antibodies, Monoclonal / immunology
Antigens, Neoplasm / immunology,  isolation & purification*
Antigens, Surface / immunology,  isolation & purification*
Cell Differentiation
Cell Line
Female
Lectins / immunology
Leukemia, Experimental / immunology*
Membrane Glycoproteins*
Mice
Mice, Inbred A
Mice, Inbred AKR
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Inbred DBA
Mice, Inbred NZB
Molecular Weight
Pregnancy
Receptors, Mitogen / isolation & purification
T-Lymphocytes / cytology,  immunology*,  pathology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/Antigens, Surface; 0/Dolichos biflorus lectin receptor; 0/Lectins; 0/Membrane Glycoproteins; 0/Receptors, Mitogen; 0/thymus-leukemia antigens
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 4 Year: 1984
Volume: 159 Issue: 4
First Page: 971 Last Page: 980
ID: 2187277
Publisher Id: 84163781
PubMed Id: 6608576

A new differentiation antigen (FT-1) shared with fetal thymocytes and leukemic cells in the mouse


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