Document Detail

A new continuous-flow cell separation method based on cell density: principle, apparatus, and preliminary application to separation of human buffy coat.
MedLine Citation:
PMID:  11835415     Owner:  NLM     Status:  MEDLINE    
With the recent progress in transfusion medicine, separation and isolation of cells in a large quantity is becoming increasingly important. At present, the continuous cell separation method in a preparative scale is limited to apheresis and elutriation: the former is mainly used for collection of platelet and buffy coat from the whole blood, while the latter separates cells virtually according to their size. Here we introduce a continuous flow method that separates cells entirely based on cell density. The method is gentle and capable of processing a large number of cells. The potential capability of the method was demonstrated on separation of lymphocytes and granulocytes from human buffy coat. Lymphocytes were enriched to 90% in the fraction at density = 1.065 and granulocytes are isolated in fractions at density = 1.075-1.080 while red cells were completely retained at the periphery of the channel. CD34 cells were distributed around 1.065 and coeluted with lymphocytes, suggesting that further enrichment requires focusing the density gradient around 1.070. The method could process 10(9) nucleated cells in 2 hours. Our preliminary results suggest that the present method is an effective and efficient means to separate blood cells.
Y Ito; K Shinomiya
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical apheresis     Volume:  16     ISSN:  0733-2459     ISO Abbreviation:  J Clin Apher     Publication Date:  2001  
Date Detail:
Created Date:  2002-02-08     Completed Date:  2002-05-06     Revised Date:  2005-07-26    
Medline Journal Info:
Nlm Unique ID:  8216305     Medline TA:  J Clin Apher     Country:  United States    
Other Details:
Languages:  eng     Pagination:  186-91     Citation Subset:  IM    
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1676, USA.
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MeSH Terms
Blood Cell Count
Cell Separation / instrumentation,  methods*
Centrifugation, Density Gradient
Equipment Design
Hematopoietic Stem Cells
Indicators and Reagents
Silicon Dioxide
Reg. No./Substance:
0/Indicators and Reagents; 65455-52-9/Percoll; 7631-86-9/Silicon Dioxide; 9003-39-8/Povidone

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