| A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of lambda cro binding heptadecanucleotide on a polymer support. | |
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MedLine Citation:
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PMID: 7177845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new condensing reagent 1-(2,4,6,-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide (TPSPy) was found to give one diastereoisomer of dinucleoside monophosphate aryl esters. Several oligodeoxynucleotide blocks were prepared using this reagent. A heptadecanucleotide, dTATCCCTTGCGGTGATA, which had the same sequence as the lambda cro binding DNA sequence was synthesized by condensing mono-, tri- and dodecanucleotide blocks using this reagent on a polystyrene support. |
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Authors:
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E Ohtsuka; Z Tozuka; S Iwai; M Ikehara |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleic acids research Volume: 10 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1982 Oct |
Date Detail:
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Created Date: 1983-02-14 Completed Date: 1983-02-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 6235-41 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Azoles* Base Sequence Chromatography, Thin Layer Indicators and Reagents* Magnetic Resonance Spectroscopy Polydeoxyribonucleotides / chemical synthesis* Tetrazoles* |
| Chemical | |
Reg. No./Substance:
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0/Azoles; 0/Indicators and Reagents; 0/Polydeoxyribonucleotides; 0/Tetrazoles; 81186-95-0/1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 25 Month: 10 Year: 1982 Volume: 10 Issue: 20 First Page: 6235 Last Page: 6241 ID: 326913 PubMed Id: 7177845 |
| A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of lambda cro binding heptadecanucleotide on a polymer support. | |
| E Ohtsuka | |
| Z Tozuka | |
| S Iwai | |
| M Ikehara | |
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