Document Detail

A new, concise dialysis approach to assessment of cardiac sympathetic nerve terminal abnormalities.
MedLine Citation:
PMID:  9087591     Owner:  NLM     Status:  MEDLINE    
We applied a dialysis technique to the hearts of anesthetized cats and examined whether the concentration of dialysate norepinephrine (NE) reflected NE disposition at the cardiac sympathetic nerve terminals. Dialysis probes were implanted in the left ventricular wall, and dialysate NE concentrations were measured as an index of myocardial interstitial NE levels. Stimulation of stellate ganglia significantly increased dialysate NE responses that were suppressed by local administration of an NE-releasing inhibitor (omega-conotoxin GVIA, 10 microM). Increments in basal dialysate NE levels were correlated with concentrations of a locally administered neuronal uptake blocker (desipramine; 1, 10, and 100 microM). Desipramine (100 microM) augmented stimulation-induced dialysate NE responses. Local administration of a neuronal vesicle uptake blocker (reserpine, 1 and 10 microM) did not alter dialysate NE levels but increased dialysate dihydroxyphenylglycol levels. An NE-releasing amine (tyramine, 100 microg/ml) was locally administered to examine NE storage capacity at the nerve terminal. The tyramine-induced NE-releasing response was completely abolished by pretreatment with reserpine (1 mg/kg i.p.). Thus cardiac dialysis with local administration of a pharmacological tool offers a new, concise approach to assessment of neuronal NE release, uptake, vesicle uptake, and storage capacity by cardiac sympathetic nerve terminals.
T Yamazaki; T Akiyama; H Kitagawa; Y Takauchi; T Kawada; K Sunagawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  272     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-04-28     Completed Date:  1997-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1182-7     Citation Subset:  IM    
Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
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MeSH Terms
Calcium Channel Blockers / pharmacology
Chromatography, High Pressure Liquid
Desipramine / pharmacology
Dialysis / methods
Efferent Pathways / physiology*
Electric Stimulation
Heart / innervation*
Methoxyhydroxyphenylglycol / analogs & derivatives,  metabolism
Nerve Endings / drug effects,  physiology*
Neurons / physiology*
Norepinephrine / metabolism*
Peptides / pharmacology
Reserpine / pharmacology
Stellate Ganglion / physiology*
omega-Conotoxin GVIA
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Peptides; 3343-19-9/dihydroxyphenylethylene glycol; 50-47-5/Desipramine; 50-55-5/Reserpine; 51-41-2/Norepinephrine; 534-82-7/Methoxyhydroxyphenylglycol; 92078-76-7/omega-Conotoxin GVIA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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