| A new complementing cell line for replication-incompetent E1-deleted adenovirus propagation. | |
| | |
MedLine Citation:
|
PMID: 19002883 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
Recombinant adenoviruses (Ad) are being explored as promising delivery systems for gene therapy and vaccination. However, there is a concern about the possibility of generating replication-competent adenoviruses (RCA) using the human embryonic kidney 293 cell line. We have constructed a new cell line named the UR cell line which can be used to produce Ad vectors free of RCA. This cell line is based on the human embryonic lung HEL 299 cell. We first constructed a shuttle plasmid which encodes the E1A/E1B sequence that is necessary for adenovirus replication. The shuttle plasmid was then transfected into HEL 299 cells. The presence of the E1A/E1B sequence and protein expression in the stably transformed UR cells was confirmed. Viruses produced in UR cells were still RCA-free after ten test passages, while adenovirus produced in 293 cells had generated RCA during the fourth passage. We conclude that the UR cell line is sufficiently stable, can effectively produce a virus yield comparable with 293 cells, and does not generate RCA formation during Ad propagation. |
| | |
Authors:
|
Qingfu Xu; Maria T Arevalo; Michael E Pichichero; Mingtao Zeng |
Related Documents
:
|
2320583 - Overexpressed human metallothionein iia gene protects chinese hamster ovary cells from ... 8624413 - Cdna cloning and gene expression of ascorbate oxidase in tobacco. 4055263 - Rat pleurisy induced by kaolin or croton oil: time course of fluid accumulation and whi... |
Publication Detail:
|
Type: Journal Article Date: 2006-11-18 |
Journal Detail:
|
Title: Cytotechnology Volume: 51 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 2006 Jul |
Date Detail:
|
Created Date: 2008-11-12 Completed Date: 2011-07-14 Revised Date: 2013-02-19 |
Medline Journal Info:
|
Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 133-40 Citation Subset: - |
Affiliation:
|
Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Box 672, Rochester, NY, 14642, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intracellular nucleotide pools and ratios as tools for monitoring dedifferentiation of primary porci...
Next Document: Optimization of an electroporation protocol using the K562 cell line as a model: role of cell cycle ...