| A new competitive enzyme-linked immunosorbent assay (ELISA) for determination of estrogenic bisphenols. | |
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MedLine Citation:
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PMID: 18968727 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Bisphenol A and other hydroxylated diphenylalkanes (generally known as bisphenols) have been identified as potential estrogenic substances. In this paper, a specific polyclonal antibody was produced against these compounds by immunization of rabbits with a conjugate of 4,4-bis (4-hydroxyphenyl) valeric acid and bovine serum albumin (BHPVA-BSA). The polyclonal antibody showed specific recognition of the bisphenol structure, while the cross reactions of other common phenolic compounds such as phenol, hydroquinol and p-hydroxybenzoic acid were all lower than 1%. The linear range of bisphenol A calibration curve was 1-10 000 ng ml(-1). Real water samples and mouse serum samples were spiked with known amount of bisphenol A and measured by the competitive ELISA. Recoveries were between 92 and 105%. The detection limits were found to be 0.1 ng ml(-1) for real water samples and 2 ng ml(-1) for serum samples. The method is very useful for monitoring bisphenol compounds in environmental and biological samples. |
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Authors:
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Mei-Ping Zhao; Yuan-Zong Li; Zhen-Quan Guo; Xin-Xiang Zhang; Wen-Bao Chang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Talanta Volume: 57 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2008-10-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 1205-10 Citation Subset: - |
Affiliation:
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The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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