Document Detail


A new competitive enzyme-linked immunosorbent assay (ELISA) for determination of estrogenic bisphenols.
MedLine Citation:
PMID:  18968727     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Bisphenol A and other hydroxylated diphenylalkanes (generally known as bisphenols) have been identified as potential estrogenic substances. In this paper, a specific polyclonal antibody was produced against these compounds by immunization of rabbits with a conjugate of 4,4-bis (4-hydroxyphenyl) valeric acid and bovine serum albumin (BHPVA-BSA). The polyclonal antibody showed specific recognition of the bisphenol structure, while the cross reactions of other common phenolic compounds such as phenol, hydroquinol and p-hydroxybenzoic acid were all lower than 1%. The linear range of bisphenol A calibration curve was 1-10 000 ng ml(-1). Real water samples and mouse serum samples were spiked with known amount of bisphenol A and measured by the competitive ELISA. Recoveries were between 92 and 105%. The detection limits were found to be 0.1 ng ml(-1) for real water samples and 2 ng ml(-1) for serum samples. The method is very useful for monitoring bisphenol compounds in environmental and biological samples.
Authors:
Mei-Ping Zhao; Yuan-Zong Li; Zhen-Quan Guo; Xin-Xiang Zhang; Wen-Bao Chang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Talanta     Volume:  57     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2008-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  1205-10     Citation Subset:  -    
Affiliation:
The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China.
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