| A new cleavage site for elastase within the complement component 3. | |
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MedLine Citation:
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PMID: 20854470 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lysosomal enzyme elastase was earlier shown to cleave the complement molecule C3. During some preliminary experiments on the interactions of certain pathogenic bacteria with the innate defence mechanisms, we observed C3 cleavage, in the presence of elastase, to fragments not previously described. To elucidate this proteolytic reaction, the present study was conducted. Degradation of C3 in mixtures with elastase or cathepsin G was detected by an immunoblot procedure using anti-C3c and anti-C3d antibodies after separating the proteins by SDS-PAGE. Certain C3 fragments were analysed for amino acid sequence. The results revealed the existence of a cleavage site for elastase at the position alanine1350/lysine1351 of the C3 molecule, which has not been previously described. The fragment resulted from this cleavage has a size of about 39 kDa and it contains a part or the whole of C3d. This cleavage was distinct from the one previously described at position 987/988, which gives a 34 kDa C3d-containing fragment. |
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Authors:
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Rolf Claesson; Eleni Kanasi; Anders Johansson; Sotirios Kalfas |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 118 ISSN: 1600-0463 ISO Abbreviation: APMIS Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-21 Completed Date: 2010-10-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8803400 Medline TA: APMIS Country: Denmark |
Other Details:
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Languages: eng Pagination: 765-8 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal Compilation © 2010 APMIS. |
Affiliation:
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Department of Odontology, Umeå University, Umeå, Sweden. rolf.claesson@odont.umu.se |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Complement C3
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metabolism* Electrophoresis, Polyacrylamide Gel Humans Immunoblotting Pancreatic Elastase / metabolism* Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
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0/Complement C3; EC 3.4.21.36/Pancreatic Elastase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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