Document Detail


A new biomechanical perfusion system for ex vivo study of small biological intact vessels.
MedLine Citation:
PMID:  16389529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The vascular endothelium transduces physical stimuli within the circulation into physiological responses, which influence vascular remodelling and tissue homeostasis. Therefore, a new computerized biomechanical ex vivo perfusion system was developed, in which small intact vessels can be perfused under well-defined biomechanical forces. The system enables monitoring and regulation of vessel lumen diameter, shear stress, mean pressure, variable pulsatile pressure and flow profile, and diastolic reversal flow. Vessel lumen measuring technique is based on detection of the amount of flourescein over a vessel segment. A combination of flow resistances, on/off switches, and capacitances creates a wide range of pulsatile pressures and flow profiles. Accuracy of the diameter measurement was evaluated. The diameters of umbilical arteries were measured and compared with direct ultrasonographic measurement of the vessel diameter. As part of the validation the pulsatile pressure waveform was altered, e.g., in terms of pulse pressure, frequency, diastolic shape, and diastolic reversal flow. In a series of simulation experiments, the hemodynamic homeostasis functions of the system were successfully challenged by generating a wide range of vascular diameters in artificial and intact human vessels. We conclude that the system presented may serve as a methodological and technical platform when performing advanced hemodynamic stimulation protocols.
Authors:
Niklas Bergh; Mikael Ekman; Erik Ulfhammer; Maria Andersson; Lena Karlsson; Sverker Jern
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  33     ISSN:  0090-6964     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2006-01-03     Completed Date:  2006-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1808-18     Citation Subset:  IM    
Affiliation:
Clinical Experimental Research Laboratory, Heart and Lung Institute, Sahlgrenska University Hospital/Ostra, Göteborg University, Göteborg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Vessels / anatomy & histology,  physiology*
Diagnostic Imaging* / instrumentation,  methods
Endothelium, Vascular / physiology*
Homeostasis / physiology*
Humans
Mechanotransduction, Cellular / physiology*
Organ Culture Techniques
Perfusion* / instrumentation,  methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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