| A new biodegradable poly-amino acid: alpha,beta-poly[(N-hydroxypropyl/aminoethyl)-DL-aspartamide-co-L-lysine], a potential nonviral vector for gene delivery. | |
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MedLine Citation:
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PMID: 15828133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new class of biodegradable poly-amino acid, alpha,beta-poly[(N-hydroxypropyl/aminoethyl)-DL-Aspartamide-co-L-Lysine] (PHAAL), was synthesized by ring-opening of poly[succinimide-co-lysine](PSL) with n-propanolamine and ethylene diamine after thermal copolycondensation of DL-Aspartic acid and L-lysine under reduced pressure. Different ratio feeds of PSL were obtained and characterized by 1H-NMR, Fourier transformed infrared spectroscopy, X-ray, thermogravimetric analysis and gel permeation chromatography experiments. As one of the polycationic materials, performed for gene delivery carrier, the PHAAL degradation experiment was carried out in PBS (10 mM, pH =7.4) and enzyme (papain, trypsine 1 mg/ml, 37 +/- 0.1 degree C) solution. PHAAL had lower cytotoxicity than polyethylenimine (25KDa) and poly-L-Lysine (30 KDa), in Hela, E.C.V.-304, Bcap 37 cell lines. Particle size and zeta, potential of PHAAL/DNA complexes were measured. Sizes ranged from 300-500 nm and zeta potentials were at -20 to 2,5 mV. The condensation ability of PHAAL for DNA was evaluated by agarose gel electrophoresis. The PHAAL could completely neutralize DNA at N/P ratio (w/w) 150:1. |
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Authors:
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Yangzhi Guping; Tang Guping; Yànjie |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Drug delivery Volume: 12 ISSN: 1071-7544 ISO Abbreviation: Drug Deliv Publication Date: 2005 Mar-Apr |
Date Detail:
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Created Date: 2005-04-13 Completed Date: 2005-07-14 Revised Date: 2006-04-21 |
Medline Journal Info:
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Nlm Unique ID: 9417471 Medline TA: Drug Deliv Country: England |
Other Details:
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Languages: eng Pagination: 89-96 Citation Subset: IM |
Affiliation:
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College of Pharmaceutical Science, Zhejiang University, 117602 Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aspartic Acid
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administration & dosage,
genetics,
pharmacokinetics Biopolymers Biotransformation Drug Delivery Systems / methods* Gene Therapy / methods* Genetic Vectors / administration & dosage* Hela Cells Humans Peptides / administration & dosage, genetics, pharmacokinetics Polylysine / administration & dosage, genetics, pharmacokinetics Proteins / administration & dosage, genetics, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Biopolymers; 0/Peptides; 0/Proteins; 0/alpha,beta-poly(aspartic acid); 25104-18-1/Polylysine; 26063-13-8/polyaspartate; 56-84-8/Aspartic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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