| A new automatic device for routine cord blood banking: critical analysis of different volume reduction methodologies. | |
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MedLine Citation:
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PMID: 19929473 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AIMS: Volume reduction is the usual process in cord blood banking that has some advantages regarding reducing the storage space and dimethyl sulfoxide (DMSO) quantity in the final product. The volume reduction methodology must guarantee high cell recovery and red blood cell (RBC) depletion by reducing all the umbilical cord blood (UCB) units to a standard volume. METHODS: We analyzed and compared critically three different volume reduction methods [hydroxyethylstarch (HES), top and bottom with Optipress II and Compomat G4, and AXP] used at the Valencia Cord Blood Bank over 10 years. RESULTS: The highest significant RBC depletion was achieved with the AXP system (P<0.001), while the top and bottom system with Compomat G4 and an adjusted buffy coat (BC) volume to 41 mL enabled the best total nucleated cell (TNC) recovery (P<0.001). TNC recovery and RBC depletion were similar for AXP and HES with an adjusted volume to 21 mL. In the multivariate analysis, when analyzing all cases, the BC volume set significantly influenced TNC, CD34+ and lymphocyte recoveries and RBC depletion (P<0.001). RBC depletion was significantly influenced by the initial volume and initial RBC content of UCB units (P<0.001). CONCLUSIONS: AXP is a highly efficient method for RBC depletion, providing the same TNC recovery as HES method with a final volume of 41 mL. AXP has the advantages of being an automatic and functionally closed system that shortens and better standardizes the proceedings. Top and bottom is a closed system that allows better TNC recoveries when the BC volume set is 41 mL. |
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Authors:
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Pilar Solves; Vicente Mirabet; Amando Blanquer; Francisco Delgado-Rosas; Dolores Planelles; Margarita Andrade; Francisco Carbonell-Uberos; M Angeles Soler; Roberto Roig |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytotherapy Volume: 11 ISSN: 1477-2566 ISO Abbreviation: Cytotherapy Publication Date: 2009 |
Date Detail:
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Created Date: 2009-11-25 Completed Date: 2010-01-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100895309 Medline TA: Cytotherapy Country: England |
Other Details:
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Languages: eng Pagination: 1101-7 Citation Subset: IM |
Affiliation:
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Cord Blood Bank Transfusion Center, Valencia, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Automation
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instrumentation* Blood Banks / methods* Cell Nucleus / metabolism Cell Size* Erythrocytes / cytology* Fetal Blood / cytology* Humans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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