Document Detail

A new assay of X-prolyl dipeptidyl-aminopeptidase activity in human serum with glycylproline p-phenylazoanilide as substrate.
MedLine Citation:
PMID:  37425     Owner:  NLM     Status:  MEDLINE    
A new assay procedure for X-prolyl dipeptidyl-aminopeptidase activity in human serum was developed with glycylproline p-phenylazoanilide tosylate as substrate. p-Phenylazoaniline liberated by the enzyme reaction was measured photometrically at 493 nm after stopping the reaction with acid. This assay was simple and sensitive, and less than 50 microliter of human serum was required for the assay. Km value was 2.5 mM and the optimum pH was 8.7. After disc gel electrophoresis of human serum, the enzyme activity could be distinctly observed as a reddish band on the gel when the gel was incubated with this substrate.
T Kato; K Iwase; T Nagatsu; M Hino; T Takemoto; S Sakakibara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  24     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1979 Mar 
Date Detail:
Created Date:  1979-09-25     Completed Date:  1979-09-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  9-13     Citation Subset:  IM    
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MeSH Terms
Electrophoresis, Disc
Endopeptidases / blood*
Hydrogen-Ion Concentration
Proline / analogs & derivatives
Reg. No./Substance:
0/Dipeptides; 147-85-3/Proline; EC 3.4.-/Endopeptidases

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