| A new approach to heart valve tissue engineering: mimicking the heart ventricle with a ventricular assist device in a novel bioreactor. | |
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MedLine Citation:
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PMID: 20687125 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The 'biomimetic' approach to tissue engineering usually involves the use of a bioreactor mimicking physiological parameters whilst supplying nutrients to the developing tissue. Here we present a new heart valve bioreactor, having as its centrepiece a ventricular assist device (VAD), which exposes the cell-scaffold constructs to a wider array of mechanical forces. The pump of the VAD has two chambers: a blood and a pneumatic chamber, separated by an elastic membrane. Pulsatile air-pressure is generated by a piston-type actuator and delivered to the pneumatic chamber, ejecting the fluid in the blood chamber. Subsequently, applied vacuum to the pneumatic chamber causes the blood chamber to fill. A mechanical heart valve was placed in the VAD's inflow position. The tissue engineered (TE) valve was placed in the outflow position. The VAD was coupled in series with a Windkessel compliance chamber, variable throttle and reservoir, connected by silicone tubings. The reservoir sat on an elevated platform, allowing adjustment of ventricular preload between 0 and 11 mmHg. To allow for sterile gaseous exchange between the circuit interior and exterior, a 0.2 µm filter was placed at the reservoir. Pressure and flow were registered downstream of the TE valve. The circuit was filled with culture medium and fitted in a standard 5% CO(2) incubator set at 37 °C. Pressure and flow waveforms were similar to those obtained under physiological conditions for the pulmonary circulation. The 'cardiomimetic' approach presented here represents a new perspective to conventional biomimetic approaches in TE, with potential advantages. Copyright © 2010 John Wiley & Sons, Ltd. |
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Authors:
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Andreas Kaasi; Idágene A Cestari; Noedir A G Stolf; Adolfo A Leirner; Ole Hassager; Ismar N Cestari |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of tissue engineering and regenerative medicine Volume: 5 ISSN: 1932-7005 ISO Abbreviation: J Tissue Eng Regen Med Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101308490 Medline TA: J Tissue Eng Regen Med Country: England |
Other Details:
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Languages: eng Pagination: 292-300 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 John Wiley & Sons, Ltd. |
Affiliation:
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Heart Institute (InCor), University of São Paulo Medical School, Bioengineering Division, São Paulo, Brazil; M.Sc. (Eng) Program, Medicine and Technology, Technical University of Denmark and University of Copenhagen, Denmark; Danish Polymer Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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