Document Detail

A new approach to the biochemical pathology of the vascular system, using time governed laminar elution and bioluminescence analyses.
MedLine Citation:
PMID:  3807899     Owner:  NLM     Status:  MEDLINE    
Endothelial cells which cover the inner wall of blood vessels were extracted for bioluminescence analyses of nucleotides and enzymes. The contaminating blood was removed by heparinization and rinsing with ammonium chloride. The content of the endothelial cells of the rat aorta was reached by time governed laminar elution, using a saponin solution to disrupt the cell membranes. Uniformity of extraction was achieved with a Hamilton programmable pump. All the analyses of the eluted fractions showed a characteristic and reproducible peak. The activities of glucose-6-phosphate dehydrogenase and adenylate kinase were significantly higher in the diabetic animals whereas the amount of nucleotides did not differ between diabetic and control rats. The laminar elution technique combined with bioluminescence assay represents a new approach to studies of biochemical alterations in the endothelial cells. The method is also useful for extraction and analyses of other surface layers.
S E Brolin; P Naeser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  73     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-03-11     Completed Date:  1987-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  55-9     Citation Subset:  IM    
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MeSH Terms
Adenine Nucleotides / analysis
Adenylate Kinase / analysis
Diabetes Mellitus, Experimental / enzymology,  metabolism*
Endothelium / enzymology,  metabolism*
Glucosephosphate Dehydrogenase / analysis
Hemoglobins / analysis
Luminescent Measurements
Malate Dehydrogenase / analysis
Nucleotides / analysis
Reg. No./Substance:
0/Adenine Nucleotides; 0/Hemoglobins; 0/Nucleotides; EC Dehydrogenase; EC Dehydrogenase; EC Kinase

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