| A new approach to amino acid racemization in enamel: testing of a less destructive sampling methodology. | |
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MedLine Citation:
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PMID: 18537866 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aspartic acid racemization has been found to be an accurate measure of age at death for recent forensic material. This paper examines the practicality of using acid etching of the tooth surface to extract amino acids from the enamel for racemization analysis. By serial etching of the tooth and contamination of the teeth with bovine serum albumin prior to etching, the ability of etching to remove contamination was assessed. The destructiveness of the method was visualized and quantified using micro-computed tomography (micro-CT). By bleaching the teeth and by deeper etching it was possible to obtain more consistent values. While etching had little effect on the enamel at the macroscale, it did have an impact at the microscale. The quantities of enamel removed varied depending upon the tooth morphology, but were not large. Acid etching of enamel thus appears to be a promising new method for extracting proteins for amino acid racemization age estimation noninvasively. |
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Authors:
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Rebecca C Griffin; Howard Moody; Kirsty E H Penkman; Michael J Fagan; Neil Curtis; Matthew J Collins |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of forensic sciences Volume: 53 ISSN: 1556-4029 ISO Abbreviation: J. Forensic Sci. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-21 Completed Date: 2008-11-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375370 Medline TA: J Forensic Sci Country: United States |
Other Details:
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Languages: eng Pagination: 910-6 Citation Subset: IM |
Affiliation:
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BioArch, Department of Archaeology, University of York, YO10 5DD, UK. r.griffin@liverpool.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Determination by Teeth
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methods* Amino Acids / analysis* Dental Enamel / chemistry*, radiography Dental Etching* Disinfectants Forensic Dentistry / methods* Humans Sodium Hypochlorite Stereoisomerism Tomography, X-Ray Computed |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Disinfectants; 7681-52-9/Sodium Hypochlorite |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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