Document Detail


A new apparatus for studying the effect of hydrostatic pressure on cells in culture : application to osteoblastic cells ROS 17/2.8.
MedLine Citation:
PMID:  22358604     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Although mechanical stresses have long been recognized as an important factor in the regulation of bone remodeling, the mechanism underlying this effect has remained obscure. A number of methods have been devised to apply forces to bone tissues and bone-derived cells in order to investigate the biochemical results of mechanical stimuli. In this paper we report a method for applying a well controlled cyclic hydrostatic pressure on cultured ROS 17/2.8 osteoblastic lineage cells. This technique allows the investigation of the true frequency response of cells. Hydrostatic pressure with a 1 Hz frequency decreases alkaline phosphatase activity of confluent osteoblastic-like cells (ROS 17/2.8).
Authors:
L Vergne; A Meunier; M Adolphe; L Sedel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  21     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1996 Jan 
Date Detail:
Created Date:  2012-02-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  21-30     Citation Subset:  -    
Affiliation:
Laboratoire de Recherches Orthopédiques, Université Paris VII Denis Diderot, URA CNRS 1432, Faculté de Médecine Lariboisière Saint-Louis, 10 Avenue de Verdun, 75010, Paris, France.
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