Document Detail


A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations.
MedLine Citation:
PMID:  343917     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
L-cells synchronized by mitotic selection were investigated by flow-cytometry and the fractions of cells in the various cell cycle compartments were determined as a function of time. A new analytical evaluation procedure was developed, by which the mean transit-times of cells through various cell cycle phases can be calculated from these data. Three examples for application of the method are presented: (1) determination of the duration of G1, S, G2 + M and of the whole cell cycle; (2) calculation of the rate of DNA synthesis in several subcompartments of the S-phase; and (3) evaluation of the degree of synchronization at different stages of the cell cycle.
Authors:
H P Beck
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  11     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1978 Mar 
Date Detail:
Created Date:  1978-05-17     Completed Date:  1978-05-17     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  139-48     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle*
Cytological Techniques*
DNA / biosynthesis
Interphase
Kinetics
L Cells (Cell Line)
Mathematics
Mitosis
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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