| A new amperometric bienzymatic biosensor based on biocomposites for the determination of gluconic acid in wines. | |
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MedLine Citation:
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PMID: 21726760 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A new amperometric bienzymatic biosensor for gluconic acid based on the coimmobilization of gluconokinase (EC 2.7.1.12) and phosphogluconate dehydrogenase (EC 1.1.1.44) by polysulfone membrane entrapment onto the surface of a graphite-epoxy composite is reported. This biosensor represents an alternative to gluconate dehydrogenase (EC 1.1.99.3) based methods, which is no longer commercially available. Measurements were done at an applied potential of +0.800V, room temperature and phosphate buffer pH 7.50; obtaining a linear response range for gluconic acid extended from 7.0×10(-6) to 2.5×10(-4)M. Constructed biosensors showed good reproducibility for calibrations using different electrodes (RSD of 1.74%). Finally, biosensor was applied to real wine samples, and the results obtained were validated by comparison with those provided by a reference laboratory. Good correlation was found when the biosensor results were plotted vs. the reference values (slope=1.03±0.04, intercept=0.01±0.02, r(2)=0.995). |
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Authors:
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Xavier Cetó; Francisco Céspedes; Josefina Capdevila; Manel Del Valle |
Publication Detail:
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Type: Journal Article Date: 2011-05-30 |
Journal Detail:
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Title: Talanta Volume: 85 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 1207-12 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Sensors and Biosensors Group, Department of Chemistry, Universitat Autònoma de Barcelona, Edifici Cn, 08193 Bellaterra, Barcelona, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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