Document Detail

A new algorithm for deduction of hepatitis B surface antigen subtype determinants from the amino acid sequence.
MedLine Citation:
PMID:  17164557     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: We have reexamined hepatitis B virus subtypes to determine the role of specific HBsAg amino acids in serologic reactivity because of problematic genotype/subtype associations seen in a set of geographically diverse serum specimens. METHODS: We obtained DNA sequences for 491 HBsAg-positive specimens from geographically distinct locations, determined their genotypes through phylogenetic analysis, and subtyped the specimens using an algorithm derived from published data on the molecular basis of HBsAg subtype reactivity. Problematic samples were subtyped serologically to resolve conflicts based on the amino acid sequence alone. RESULTS: Three isolates were found to have unusual genotype/subtype associations. Examination of the isolates' amino acid sequences suggested amino acid positions 122, 127, 140, 159 and 160 can be used to determine subtype reactivity from HBsAg amino acid sequences, while position 134, previously thought to play a role, is no longer important. CONCLUSIONS: This re-examination of hepatitis B virus subtypes shows the involvement of amino acid positions 122, 127, 140, 159 and 160 in HBsAg reactivity. While d, y, and r reactivities are controlled by single amino acid changes, w reactivity is determined by positions 122, 127, 140, and 159.
Michael A Purdy; Ganesh Talekar; Paul Swenson; Aufra Araujo; Howard Fields
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Publication Detail:
Type:  Journal Article     Date:  2006-11-24
Journal Detail:
Title:  Intervirology     Volume:  50     ISSN:  0300-5526     ISO Abbreviation:  Intervirology     Publication Date:  2007  
Date Detail:
Created Date:  2006-12-13     Completed Date:  2007-02-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364265     Medline TA:  Intervirology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  45-51     Citation Subset:  IM    
Copyright Information:
Copyright 2007 S. Karger AG, Basel.
Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
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MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal / immunology
Hepatitis B / virology
Hepatitis B Surface Antigens / chemistry*,  immunology
Hepatitis B virus / classification*,  genetics*,  immunology
Molecular Sequence Data
Sequence Alignment
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Hepatitis B Surface Antigens

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