Document Detail


A new algorithm for deduction of hepatitis B surface antigen subtype determinants from the amino acid sequence.
MedLine Citation:
PMID:  17164557     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: We have reexamined hepatitis B virus subtypes to determine the role of specific HBsAg amino acids in serologic reactivity because of problematic genotype/subtype associations seen in a set of geographically diverse serum specimens. METHODS: We obtained DNA sequences for 491 HBsAg-positive specimens from geographically distinct locations, determined their genotypes through phylogenetic analysis, and subtyped the specimens using an algorithm derived from published data on the molecular basis of HBsAg subtype reactivity. Problematic samples were subtyped serologically to resolve conflicts based on the amino acid sequence alone. RESULTS: Three isolates were found to have unusual genotype/subtype associations. Examination of the isolates' amino acid sequences suggested amino acid positions 122, 127, 140, 159 and 160 can be used to determine subtype reactivity from HBsAg amino acid sequences, while position 134, previously thought to play a role, is no longer important. CONCLUSIONS: This re-examination of hepatitis B virus subtypes shows the involvement of amino acid positions 122, 127, 140, 159 and 160 in HBsAg reactivity. While d, y, and r reactivities are controlled by single amino acid changes, w reactivity is determined by positions 122, 127, 140, and 159.
Authors:
Michael A Purdy; Ganesh Talekar; Paul Swenson; Aufra Araujo; Howard Fields
Related Documents :
16101307 - Identification in the mold hypocrea jecorina of the first fungal d-galacturonic acid re...
18022727 - Susceptibility to type 1 autoimmune hepatitis is associated with shared amino acid sequ...
10737147 - Large alkyl side-chains of isoleucine and leucine in the npirl region constitute the co...
1554737 - Koningic acid (heptelidic acid) inhibition of glyceraldehyde-3-phosphate dehydrogenases...
20600307 - Long-chain polyunsaturated fatty acids: selected mechanisms of action on bone.
10095997 - Regulatory interactions between lipids and carbohydrates: the glucose fatty acid cycle ...
Publication Detail:
Type:  Journal Article     Date:  2006-11-24
Journal Detail:
Title:  Intervirology     Volume:  50     ISSN:  0300-5526     ISO Abbreviation:  Intervirology     Publication Date:  2007  
Date Detail:
Created Date:  2006-12-13     Completed Date:  2007-02-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364265     Medline TA:  Intervirology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  45-51     Citation Subset:  IM    
Copyright Information:
Copyright 2007 S. Karger AG, Basel.
Affiliation:
Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. MPurdy@cdc.gov
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Algorithms*
Amino Acid Sequence
Antibodies, Monoclonal / immunology
Genotype
Hepatitis B / virology
Hepatitis B Surface Antigens / chemistry*,  immunology
Hepatitis B virus / classification*,  genetics*,  immunology
Humans
Molecular Sequence Data
Sequence Alignment
Serotyping
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Hepatitis B Surface Antigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Analysis of repeat units in the R2 region among different Oka varicella-zoster virus vaccine strains...
Next Document:  Molecular epidemiology of hepatitis B virus (HBV) genotypes and serotypes in patients with chronic H...