| A new Schizosaccharomyces pombe chronological lifespan assay reveals that caloric restriction promotes efficient cell cycle exit and extends longevity. | |
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MedLine Citation:
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PMID: 19409973 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a new chronological lifespan (CLS) assay for the yeast Schizosaccharomyces pombe. Yeast CLS assays monitor the loss of cell viability in a culture over time, and this new assay shows a continuous decline in viability without detectable regrowth until all cells in the culture are dead. Thus, the survival curve is not altered by the generation of mutants that can grow during the experiments, and one can monitor the entire lifespan of a strain until the number of viable cells has decreased over 10(6)-fold. This CLS assay recapitulates the evolutionarily conserved features of lifespan shortening by over nutrition, lifespan extension by caloric restriction, increased stress resistance of calorically restricted cells and lifespan control by the AKT kinases. Both S. pombe AKT kinase orthologs regulate CLS: loss of sck1(+) extended lifespan in over nutrition conditions, loss of sck2(+) extended lifespan under both normal and over nutrition conditions, and loss of both genes showed that sck1(+) and sck2(+) control different longevity pathways. The longest-lived S. pombe cells showed the most efficient cell cycle exit, demonstrating that caloric restriction links these two processes. This new S. pombe CLS assay will provide a valuable tool for aging research. |
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Authors:
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Bo-Ruei Chen; Kurt W Runge |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-05-04 |
Journal Detail:
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Title: Experimental gerontology Volume: 44 ISSN: 1873-6815 ISO Abbreviation: Exp. Gerontol. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-27 Completed Date: 2011-01-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
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Languages: eng Pagination: 493-502 Citation Subset: IM |
Affiliation:
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Department of Molecular Genetics, Cleveland Clinic Lerner College of Medicine at CWRU, 9500 Euclid Avenue NE 20, Cleveland, OH 44195, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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genetics,
physiology* Caloric Restriction* Cell Cycle* Cell Survival Gene Expression Regulation, Fungal / genetics, physiology Humans Longevity / genetics, physiology* Reactive Oxygen Species Schizosaccharomyces / genetics, physiology* Schizosaccharomyces pombe Proteins / genetics |
| Grant Support | |
ID/Acronym/Agency:
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AG19660/AG/NIA NIH HHS; R01 AG019960-03/AG/NIA NIH HHS; R01 AG019960-04/AG/NIA NIH HHS; R01 AG019960-05/AG/NIA NIH HHS; R01 AG019960-06A1/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 0/Schizosaccharomyces pombe Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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