Document Detail

The neurosteroid dehydroepiandrosterone could improve somatic cell reprogramming.
MedLine Citation:
PMID:  21355850     Owner:  NLM     Status:  MEDLINE    
Expression of four major reprogramming transgenes, including Oct4, Sox2, Klf4 and c-myc, in somatic cells enables them to have pluripotency. These cells are iPSC (induced pluripotent stem cell) that currently show the greatest potential for differentiation into cells of the three germ lineages. One of the issues facing the successful reprogramming and clinical translation of iPSC technology is the high rate of apoptosis after the reprogramming process. Reprogramming is a stressful process, and the p53 apoptotic pathway plays a negative role in cell growth and self-renewal. Apoptosis via the p53 pathway serves as a major barrier in nuclear somatic cell reprogramming during iPSC generation. DHEA (dehydroepiandrosterone) is an abundant steroid that is produced at high levels in the adrenal cells, and withdrawal of DHEA increases the levels of p53 in the epithelial and stromal cells, resulting in increased levels of apoptotic cells; meanwhile, DHEA decreases cellular apoptosis. DHEA could improve the efficacy of reprogramming yield due to a decrease in apoptosis via the p53 pathway and an increase in cell viability.
Alireza Shoae-Hassani; Shiva Sharif; Javad Verdi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-21     Completed Date:  2012-01-11     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1037-41     Citation Subset:  IM    
Research Center for Science and Technology in Medicine RCSTM, Tehran University of Medical Sciences TUMS, Iran.
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MeSH Terms
Adjuvants, Immunologic / pharmacology*
Dehydroepiandrosterone / pharmacology*
Models, Theoretical
Nuclear Reprogramming / drug effects*
Pluripotent Stem Cells / cytology*,  metabolism
Tumor Suppressor Protein p53 / metabolism
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Tumor Suppressor Protein p53; 53-43-0/Dehydroepiandrosterone

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