Document Detail


The neural restrictive silencer element can act as both a repressor and enhancer of L1 cell adhesion molecule gene expression during postnatal development.
MedLine Citation:
PMID:  9501246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell adhesion molecule L1 mediates axonal guidance during neural development and mutations in its gene result in severe neurological defects. In previous studies, we identified the promoter for the L1 gene and showed that a neural restrictive silencer element (NRSE) was critical for preventing ectopic expression of L1 during early embryonic development. In the present study, we have investigated the role of the NRSE in the regulation of L1 expression during postnatal development. In gel mobility shift experiments, the NRSE formed DNA-protein complexes with nuclear extracts prepared from the brains of postnatal mice. To examine the influence of the NRSE on postnatal patterns of L1 expression in vivo, we compared the expression of two lacZ transgene constructs, one containing the native L1 gene regulatory sequences (L1lacZ) and another (L1lacZDeltaN) lacking the NRSE. Newborn mice carrying the L1lacZDeltaN showed enhanced beta-galactosidase expression relative to L1lacZ in the brain and ectopic expression in nonneural tissues. In contrast to L1lacZ mice, however, L1lacZDeltaN mice showed an unexpected loss, during postnatal development and in the adult, of beta-galactosidase expression in several neural structures, including the neural retina, cerebellum, cortex, striatum, and hippocampus. These data support the conclusion that the NRSE not only plays a role in the silencing of L1 expression in nonneural tissues during early development but also can function as a silencer and an enhancer of L1 expression in the nervous system of postnatal and adult animals.
Authors:
P Kallunki; G M Edelman; F S Jones
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  95     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-04-10     Completed Date:  1998-04-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3233-8     Citation Subset:  IM    
Affiliation:
Department of Neurobiology, The Scripps Research Institute and The Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / growth & development
Enhancer Elements, Genetic*
Gene Expression Regulation, Developmental
Genes, Reporter
Leukocyte L1 Antigen Complex
Mice
Mice, Transgenic
Nerve Tissue Proteins / biosynthesis,  genetics*
Neural Cell Adhesion Molecules / biosynthesis,  genetics*
Olfactory Pathways / growth & development
Protein Binding
Recombinant Proteins / biosynthesis
Regulatory Sequences, Nucleic Acid*
Tissue Distribution
Grant Support
ID/Acronym/Agency:
HD33576/HD/NICHD NIH HHS; NS34493/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Leukocyte L1 Antigen Complex; 0/Nerve Tissue Proteins; 0/Neural Cell Adhesion Molecules; 0/Recombinant Proteins
Comments/Corrections

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