Document Detail

The negative influence of endogenous opioid receptor activity on the differentiation of the rat pheochromocytoma PC12 cells induced by nerve growth factor.
MedLine Citation:
PMID:  9756350     Owner:  NLM     Status:  MEDLINE    
We determined the effect of naloxone and morphine on the differentiation of pheochromocytoma cell, the PC12 cells, induced by nerve growth factor (NGF). PC12 cells were grown in medium containing NGF with or without the addition of naloxone or morphine for up to 10-day treatments. NGF-induced morphological differentiation of PC12 cells was manifested by an increase in the percentage of differentiated cells and the average length of neurite per cell. Co-addition of morphine with NGF did not affect both parameters as compared to that of NGF alone. On the contrary, co-addition of naloxone with NGF significantly increased the percentage of differentiated cells, but did not affect the outgrowth of neurites. This effect of naloxone was reversed by the addition of morphine, suggesting that naloxone produced its effect by inhibiting the endogenous activity of opioid receptor. This study indicates a significant functional role of opioid receptor in NGF-induced differentiation of PC12 cells.
G C Yeh; T H Hsieh; S F Chang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  252     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-11-25     Completed Date:  1998-11-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  25-8     Citation Subset:  IM    
Graduate Institute of Medical Science, Taipei Medical College, Taiwan.
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MeSH Terms
Analgesics, Opioid / pharmacology
Cell Differentiation / drug effects
Gene Expression / drug effects
Morphine / pharmacology
Naloxone / pharmacology
Narcotic Antagonists / pharmacology
Nerve Growth Factors / pharmacology*
Neurites / drug effects,  physiology
Neurons / chemistry*,  cytology*,  ultrastructure
PC12 Cells
RNA, Messenger / metabolism
Receptors, Opioid, delta / genetics,  metabolism*
Signal Transduction / drug effects
Reg. No./Substance:
0/Analgesics, Opioid; 0/Narcotic Antagonists; 0/Nerve Growth Factors; 0/RNA, Messenger; 0/Receptors, Opioid, delta; 465-65-6/Naloxone; 57-27-2/Morphine

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