| The neem limonoids azadirachtin and nimbolide inhibit cell proliferation and induce apoptosis in an animal model of oral oncogenesis. | |
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MedLine Citation:
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PMID: 19458912 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Limonoids from the neem tree (Azadirachta indica) have attracted considerable research attention for their cytotoxicity against human cancer cell lines. However, the antiproliferative and apoptosis inducing effects of neem limonoids have not been tested in animal tumour models. The present study was therefore designed to evaluate the relative chemopreventive potential of the neem limonoids azadirachtin and nimbolide in the hamster buccal pouch (HBP) carcinogenesis model by analyzing the expression of proliferating cell nuclear antigen (PCNA), p21(waf1), cyclin D1, glutathione S-transferase pi (GST-P), NF-kappaB, inhibitor of kappaB (IkappaB), p53, Fas, Bcl-2, Bax, Bid, Apaf-1, cytochrome C, survivin, caspases-3, -6, -8 and -9, and poly(ADP-ribose) polymerase (PARP) by RT-PCR, immunohistochemical, and Western blot analyses. The results provide compelling evidence that azadirachtin and nimbolide mediate their antiproliferative effects by downregulating proteins involved in cell cycle progression and transduce apoptosis by both the intrinsic and extrinsic pathways. On a comparative basis, nimbolide was found to be a more potent antiproliferative and apoptosis inducing agent and offers promise as a candidate agent in multitargeted prevention and treatment of cancer. |
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Authors:
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G Harish Kumar; R Vidya Priyadarsini; G Vinothini; P Vidjaya Letchoumy; S Nagini |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-21 |
Journal Detail:
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Title: Investigational new drugs Volume: 28 ISSN: 1573-0646 ISO Abbreviation: Invest New Drugs Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-05-21 Completed Date: 2010-09-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309330 Medline TA: Invest New Drugs Country: United States |
Other Details:
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Languages: eng Pagination: 392-401 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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9,10-Dimethyl-1,2-benzanthracene Animals Anticarcinogenic Agents / pharmacology, therapeutic use Antineoplastic Agents, Phytogenic / pharmacology* Apoptosis / drug effects* Azadirachta* Cell Cycle Proteins / drug effects Cell Proliferation / drug effects* Cricetinae Disease Models, Animal Drug Screening Assays, Antitumor / methods Limonins / pharmacology*, therapeutic use* Male Mouth Neoplasms / chemically induced, drug therapy* Random Allocation |
| Chemical | |
Reg. No./Substance:
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0/Anticarcinogenic Agents; 0/Antineoplastic Agents, Phytogenic; 0/Cell Cycle Proteins; 0/Limonins; 11141-17-6/azadirachtin; 25990-37-8/nimbolide; 57-97-6/9,10-Dimethyl-1,2-benzanthracene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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