| The necrotroph Botrytis cinerea induces a non-host type II resistance mechanism in Pinus pinaster suspension-cultured cells. | |
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MedLine Citation:
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PMID: 18252735 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Models of non-host resistance have failed to account for the pathogenicity of necrotrophic agents. During the interaction of Pinus pinaster (maritime pine) with the non-host necrotrophic pathogen Botrytis cinerea, the generation and scavenging of reactive oxygen species (ROS) and the induction of the hypersensitive response (HR) were analyzed. Elicitation of maritime pine suspended cells with B. cinerea spores resulted in the biphasic induction of ROS. The phase I oxidative burst was dependent on calcium influx, while the phase II oxidative burst also depended on NADPH oxidase, protein kinase activity, and de novo transcription and protein synthesis. A decline was observed in catalase (CAT) and superoxide dismutase (SOD) activity, together with the down-regulation of Fe-Sod1, chlCu, Zn-Sod1 and csApx1, suggesting a coordinated response towards a decrease in the ROS-scavenging capacity of maritime pine cells during challenge. Following the second oxidative burst, programmed cell death events characteristic of the HR were observed. The results suggest the ROS-mediated and cell-breach-independent activation of Type II non-host resistance during the P. pinaster-B. cinerea interaction. |
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Authors:
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Herlânder Azevedo; Teresa Lino-Neto; Rui Manuel Tavares |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-05 |
Journal Detail:
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Title: Plant & cell physiology Volume: 49 ISSN: 1471-9053 ISO Abbreviation: Plant Cell Physiol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-11 Completed Date: 2008-06-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: Japan |
Other Details:
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Languages: eng Pagination: 386-95 Citation Subset: IM |
Affiliation:
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Grupo de Bioquímica e Fisiologia Molecular de Plantas, Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal. hazevedo@bio.uminho.pt |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants Apoptosis / physiology Botrytis / physiology* Calcium / metabolism Cells, Cultured Homeostasis / physiology Pinus / cytology*, metabolism*, microbiology Reactive Oxygen Species / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Reactive Oxygen Species; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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