Document Detail

Cachectin/tumor necrosis factor-alpha formation in human decidua. Potential role of cytokines in infection-induced preterm labor.
MedLine Citation:
PMID:  2913048     Owner:  NLM     Status:  MEDLINE    
This study was conducted as part of an investigation to evaluate the hypothesis that bacterial toxins (LPS or lipoteichoic acid), acting on macrophage-like uterine decidua to cause increased formation of cytokines, may be involved in the pathogenesis of infection-associated preterm labor. We found that cachectin/tumor necrosis factor-alpha (TNF-alpha) was synthesized and secreted into the culture medium by human decidual cells and explants in response to treatment with LPS. LPS treatment also caused an increase in PGF2 alpha production by decidual cells and explants. In amnion cells in monolayer culture, TNF-alpha stimulated PGE2 formation, and TNF-alpha was cytostatic (inhibited [3H]thymidine incorporation into DNA) but not cytolytic in amnion cells. TNF-alpha was not detectable (less than 0.34 ng/ml) in the amniotic fluid of normal pregnancies at midtrimester or at term before or after the onset of labor (n = 44); but TNF-alpha was present at concentrations between 2.8 and 22.3 ng/ml in amniotic fluids of 4 of 20 pregnancies with intact membranes complicated by preterm labor (less than 34 wk gestational age). LPS was present in 10 of the 20 amniotic fluids of preterm labor pregnancies, including all four in which TNF-alpha was present. Bacteria were identified in only one of the four LPS-positive, TNF-alpha-positive fluids. Cytokine formation in macrophage-like decidua may serve a fundamental role in the pathogenesis of preterm labor, including increased prostaglandin formation and premature rupture of the membranes.
M L Casey; S M Cox; B Beutler; L Milewich; P C MacDonald
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  83     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-03-09     Completed Date:  1989-03-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  430-6     Citation Subset:  AIM; IM    
Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas, Southwestern Medical School, Dallas 75235.
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MeSH Terms
Amniotic Fluid / analysis
Bacterial Infections / complications*
Cells, Cultured
Decidua / drug effects,  metabolism*
Dinoprost / biosynthesis
Dinoprostone / biosynthesis
Lipopolysaccharides / pharmacology
Obstetric Labor, Premature / etiology*
Pregnancy Complications, Infectious
Tumor Necrosis Factor-alpha / biosynthesis*
Grant Support
Reg. No./Substance:
0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha; 363-24-6/Dinoprostone; 551-11-1/Dinoprost

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