| The nature of high frequency sister chromatid exchange cells (HFCs). | |
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MedLine Citation:
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PMID: 9379910 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We employed the three-way differential staining technique (TWD), which allows SCEs to be distinguished on a per generation basis by scoring third metaphases (M3), in order to study the spontaneous levels of SCEs in normal and high frequency cells (HFCs) that occurred in the first (S1), second (S2) and third (S3) S phases. Fifty one of 900 lymphocytes from 37 healthy donors were defined as HFCs by calculating the 95th percentile of the distribution of SCEs in S1 + S2. 'Normal' cells presented almost the same number of SCEs after the first, second and third cell cycles (SCE averages of 2.43, 2.04 and 3.53 respectively). In contrast, HFCs showed a higher SCE count in S1, which decreased rapidly through the cycles and reached baseline level at S3 (SCE averages of 7.18, 4.29 and 3.45 respectively). This would suggest that the lesions responsible for the higher SCE frequency in HFCs were effectively removed after two cell cycles and strongly support the hypothesis that HFCs are lymphocytes which accumulate higher levels of DNA lesions through time. |
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Authors:
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I Ponzanelli; S Landi; F Bernacchi; R Barale |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mutagenesis Volume: 12 ISSN: 0267-8357 ISO Abbreviation: Mutagenesis Publication Date: 1997 Sep |
Date Detail:
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Created Date: 1997-11-10 Completed Date: 1997-11-10 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8707812 Medline TA: Mutagenesis Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 329-33 Citation Subset: IM |
Affiliation:
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Dipartimento di Scienze dell'Uomo e dell'Ambiente, Pisa University, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Cell Cycle Cells, Cultured Child Female Humans Lymphocytes / cytology* Male Middle Aged Reference Values Sex Characteristics Sister Chromatid Exchange* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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