Document Detail


The natural history of fetal cells in postpartum murine maternal lung and bone marrow: a two-stage phenomenon.
MedLine Citation:
PMID:  23128065     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During pregnancy, fetal cells cross into the maternal organs where they reside postpartum. Evidence from multiple laboratories suggests that these microchimeric fetal cells contribute to maternal tissue repair after injury. In mouse models, most injury experiments are performed during pregnancy; however, in a clinical setting most injuries or diseases occur postpartum. Therefore, experiments using animal models should be designed to address questions in the time period following delivery. In order to provide a baseline for such experiments, we analyzed the natural history of fetal cells in the postpartum maternal organs. Female C57BL/6J mice were mated to males homozygous for the enhanced green fluorescent protein gene. Fetal cells in the maternal lungs and bone marrow were identified by their green fluorescence using in a high-speed flow cytometer and their counts were compared between the lung and bone marrow. Spearman correlation analysis was used to identify relationships between the duration of time postpartum and the cell counts and ratio of live and dead cells. Our results show that fetal cells persist in these organs until at least three months postpartum in healthy female mice. We show a two-stage decline, with an initial two and a half-week rapid clearance followed by a trend of gradual decrease. Additionally, an increase in the ratio of live to dead cells within the lung over time suggests that these cells may replicate in vivo. The results presented here will inform the design of future experiments and may have implications for women's health.
Authors:
Stephanie Pritchard; Inga Peter; Kirby L Johnson; Diana W Bianchi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-01
Journal Detail:
Title:  Chimerism     Volume:  3     ISSN:  1938-1964     ISO Abbreviation:  Chimerism     Publication Date:    2012 Jul-Dec
Date Detail:
Created Date:  2013-01-09     Completed Date:  2013-06-20     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101540510     Medline TA:  Chimerism     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-64     Citation Subset:  IM    
Affiliation:
Mother Infant Research Institute, Floating Hospital at Tufts Medical Center, and Genetics Program, Department of Pathology, Sackler School of Graduate Biomedical Sciences, Boston, MA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Cells / cytology*
Chimerism*
Female
Flow Cytometry
Green Fluorescent Proteins / analysis
Lung / cytology*
Male
Maternal-Fetal Exchange
Metabolic Clearance Rate
Mice
Mice, Inbred C57BL
Postpartum Period
Pregnancy
Statistics, Nonparametric
Grant Support
ID/Acronym/Agency:
R01 HD-049469-05/HD/NICHD NIH HHS; T32 HD-049341-05/HD/NICHD NIH HHS; UL1TR000067/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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