Document Detail


The natural food grade inhibitor, lacticin 3147, reduced the incidence of mastitis after experimental challenge with Streptococcus dysgalactiae in nonlactating dairy cows.
MedLine Citation:
PMID:  10629810     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lacticin 3147 is a broad-spectrum bacteriocin produced by the food-grade organism Lactococcus lactis. Lacticin 3147 is active at a neutral pH and has been shown to be bactericidal to streptococci and staphylococci in vitro. The effectiveness of an intramammary teat seal formulation, and a teat seal containing lacticin 3147 was evaluated at drying off in 68 uninfected quarters of 18 cows. Following infusion of either teat seal or lacticin 3147 combined with teat seal, a deliberate infection challenge of Streptococcus dysgalactiae (approximately equal to 1.5 x 10(4) cfu per teat) was administered by direct inoculation into the teat sinus. During an 8-d experimental period following inoculation, 61% of control quarters and 6% of the treatment quarters either developed clinical mastitis or were shedding the challenge organism. Randomly amplified polymorphic DNA polymerase chain reaction genetic typing was used to confirm that both the new infections and the bacteria surviving in the teats at the end of the experiment were the challenge strain. The combination of teat seal and lacticin 3147 was well tolerated within the udder and elicited only a temporary increase in somatic cell count to 5.7 x 10(5)/ml (88 h after infusion) in a previously uninfected lactating udder quarter. Therefore, we concluded that this nonantibiotic approach to mastitis prevention may contribute to a reduction in the routine application of antibiotics at drying off in the future.
Authors:
M P Ryan; J Flynn; C Hill; R P Ross; W J Meaney
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  82     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-27     Completed Date:  2000-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2625-31     Citation Subset:  IM    
Affiliation:
Teagasc, Moorepark, Fermoy, Co. Cork, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / administration & dosage,  therapeutic use
Bacteriocins / therapeutic use*
Cattle
DNA Fingerprinting
Female
Hydrogen-Ion Concentration
Mammary Glands, Animal
Mastitis, Bovine / prevention & control*
Polymerase Chain Reaction
Streptococcal Infections / prevention & control*
Streptococcus / genetics
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacteriocins; 136959-47-2/lacticin 481

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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