| The natural alkaloid berberine targets multiple pathways to induce cell death in cultured human colon cancer cells. | |
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MedLine Citation:
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PMID: 22617025 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In the current paper, berberine, an isoquinoline alkaloid, was tested for its chemopreventive potential in colon cancer (SW480) cells. Berberine inhibited proliferation of colon cancer cells in a dose and time dependent manner. Interestingly, this compound exhibited minimum toxicity in normal cells at 200μM. Berberine arrested SW480 cells cycle at G2/M phase, which was supported by induction of p21 expression in the SW480 cells. Induction of a series of biochemical events, including loss of mitochondrial membrane potential, release of cytochrome-c to cytosol, induction of Bcl-2 family proteins, caspases and cleavage of Poly (ADP-ribose) polymerase (PARP), by berberine suggests its ability to induce apoptosis. In addition, berberine also inhibited inflammation, as evidenced by induction of expression of NFκB and Cox(2). Furthermore, berberine inhibited caspase-8 mediated angiogenesis, as confirmed through expression of tumor necrotic factor related apoptosis-inducing ligand (TRAIL), vascular endothelial growth factor (VEGF) and survivin. The result of the current study demonstrated that berberine has the ability to cause cell cycle arrest, induce apoptosis and inhibit inflammation in colon cancer cells. The magnitude of the effects observed suggests that berberine may be worth considering for further studies of its potential applications for improving health, either as a preventative or a potential treatment. |
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Authors:
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Kotamballi N Chidambara Murthy; Guddadarangavvanahally K Jayaprakasha; Bhimanagouda S Patil |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-19 |
Journal Detail:
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Title: European journal of pharmacology Volume: - ISSN: 1879-0712 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier B.V. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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