Document Detail


nAChR-induced octopamine release mediates the effect of nicotine onstartle response in/ofDrosophila melanogaster.
MedLine Citation:
PMID:  23331098     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Biogenic amines (BAs) play a central role in the generation of complex behaviors in vertebrates and invertebrates, including the fly Drosophila melanogaster. The comparative advantages of Drosophila as a genetic model to study the contribution of BAs to behaviors stumble upon the difficulty to access the fly brain to ask relevant physiological questions. For instance, it is not known whether the activation of nicotinic acetylcholine receptors (nAChRs) induces the release of BAs in fly brain, a phenomenon associated to several behaviors in vertebrates. Here we describe a new preparation to study the efflux of BAs in the adult fly brain by in vitrochronoamperometry. Using this preparation we show that nAChR agonists including nicotine induce a fast, transient, dose-dependent efflux of endogenous BAs, an effect mediated by α-bungarotoxin-sensitive nAChRs. By using different genetic tools we demonstrate that the BA whose efflux is induced by nAChR activation is octopamine (Oct). Furthermore, we show that theimpairment of a mechanically-induced startle response after nicotine exposure is not observed in flies deficient in Oct transmission. Thus, our data show that the efflux of BAs in Drosophila brain is increased by nAChR activation as in vertebrates, and that thenAChR-induced Oct release could have implications in a nicotine-induced behavioral response. © 2013 The Authors Journal of Neurochemistry © 2013 International Society for Neurochemistry, J. Neurochem. (2013) 10.1111/jnc.12161.
Authors:
Nicolás Fuenzalida-Uribe; Rodrigo C Meza; Hernán A Hoffmann; Rodrigo Varas; Jorge M Campusano
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-19
Journal Detail:
Title:  Journal of neurochemistry     Volume:  -     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors Journal of Neurochemistry © 2013 International Society for Neurochemistry, J. Neurochem. (2013) 10.1111/jnc.12161.
Affiliation:
Departamento de BiologíaCelular y Molecular, Pontificia Universidad Católica de Chile, Millennium Nucleus Stress and Addiction (NEDA), Portugal 49, Santiago, CHILE, 8330025.
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