| n-Hexane toxicity in Jurkat T-cells is mediated by reactive oxygen species. | |
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MedLine Citation:
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PMID: 18231777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Here we assess the role of reactive oxygen species (ROS) formation in the manifestation of n-hexane toxicity in Jurkat T-cells and the chemo-protective potential of the antioxidants epigallocatechin-3-gallate (EGCG) and thymoquinone (TQ) against n-hexane toxicity in vitro. n-Hexane is an important industrial solvent and ambient air pollutant. Subchronic exposure to n-hexane results in a concentration-dependent increase in ROS formation with a corresponding decrease in Jurkat T-cell proliferation. Results from time-course studies indicate that ROS formation plays a causal role in n-hexane induced alterations in Jurkat T-cell proliferation and membrane integrity. Treatment of cells with EGCG, at a concentration reached in plasma, reduced the ROS formation caused by exposure to n-hexane and inhibited the decrease in cell proliferation. Similar effects were obtained with TQ. Both EGCG and TQ significantly reduced n-hexane-induced LDH leakage to control levels. The combined results show that oxidative stress plays a role in the development of n-hexane toxicity. |
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Authors:
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Catherine McDermott; Maria Hutch O'Donoghue; James J A Heffron |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-30 |
Journal Detail:
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Title: Archives of toxicology Volume: 82 ISSN: 0340-5761 ISO Abbreviation: Arch. Toxicol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-04-08 Completed Date: 2008-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417615 Medline TA: Arch Toxicol Country: Germany |
Other Details:
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Languages: eng Pagination: 165-71 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Biochemical Toxicology Laboratory, University College Cork, Cork, Ireland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology Benzoquinones / pharmacology Catechin / analogs & derivatives, pharmacology Cell Proliferation / drug effects Dose-Response Relationship, Drug Hexanes / toxicity* Humans Indicators and Reagents / metabolism Jurkat Cells / drug effects, metabolism L-Lactate Dehydrogenase / metabolism Oxazines / metabolism Oxidation-Reduction Oxidative Stress / drug effects* Reactive Oxygen Species / metabolism* Solvents / toxicity* Xanthenes / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Benzoquinones; 0/Hexanes; 0/Indicators and Reagents; 0/Oxazines; 0/Reactive Oxygen Species; 0/Solvents; 0/Xanthenes; 110-54-3/n-hexane; 154-23-4/Catechin; 490-91-5/thymoquinone; 550-82-3/resazurin; 989-51-5/epigallocatechin gallate; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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