Document Detail

n-3 fatty acid deficiency decreases phosphatidylserine accumulation selectively in neuronal tissues.
MedLine Citation:
PMID:  10984109     Owner:  NLM     Status:  MEDLINE    
We have previously shown that the docosahexaenoate (22:6n-3) status in membrane phospholipids influences the biosynthesis and accumulation of phosphatidylserine (PS) in brain microsomes and C6 glioma cells. In the present study, we investigated whether the observed effect of membrane docosahexaenoic acid status on PS accumulation is universal or occurs specifically in neuronal tissues. We observed that rat brain cortex, brain mitochondria, and olfactory bulb, where 22:6n-3 is highly concentrated, contain significantly higher levels of PS in comparison to liver and adrenal, where 22:6n-3 is a rather minor component. Phospholipid molecular species analysis revealed that in brain cortex, mitochondria, and olfactory bulb 18:0,22:6n-3 was the most abundant species representing 45-65% of total PS. In nonneuronal tissues such as liver and adrenal, 18:0,20:4n-6 was the major PS species. Dietary depletion of n-3 fatty acids during prenatal and postnatal developmental periods decreased the brain 22:6n-3 content by more than 80%, with a concomitant increase in 22:5n-6 in all tissues. Under these conditions, an approximately 30-35% reduction in total PS in rat brain cortex, brain mitochondria, and olfactory bulb was observed, while PS levels in liver and adrenal were unchanged. The observed reduction of PS content in neuronal membranes appears to be due to a dramatic reduction of 18:0,22:6n-3-PS without complete replacement by 18:0,22:5n-6-PS. These results establish that variations in membrane 22:6n-3 fatty acid composition have a profound influence on PS accumulation in neuronal tissues where 22:6n-3 is abundant. These data have implications in neuronal signaling events where PS is believed to play an important role.
L Hamilton; R Greiner; N Salem; H Y Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  35     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-12-11     Completed Date:  2001-03-01     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  863-9     Citation Subset:  IM    
Section of Mass Spectrometry, Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland 20852, USA.
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MeSH Terms
Adrenal Glands / metabolism
Brain / metabolism
Chromatography, Gas
Chromatography, High Pressure Liquid
Docosahexaenoic Acids / metabolism
Fatty Acids, Omega-3 / physiology*
Liver / metabolism
Mitochondria / metabolism
Neurons / metabolism*
Olfactory Bulb / metabolism
Phosphatidylserines / biosynthesis*
Rats, Long-Evans
Spectrometry, Mass, Electrospray Ionization
Reg. No./Substance:
0/Fatty Acids, Omega-3; 0/Phosphatidylserines; 25167-62-8/Docosahexaenoic Acids

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